Introduction:

Single-cell sequencing is an emerging technique of high-throughput sequencing and analyzing genomics, transcriptome, and epigenomics at the single-cell level. Since the advent of single-cell transcriptome sequencing in 2009, diverse methodologies have been developed. These recent advances in single-cell techniques have provided a way to investigate complex biological questions without any prior information. The immune system is one such mysterious system, with a network of cells that function in various pathophysiological pathways. With the help of the 10x Genomics single-cell platform, it is now possible to study this complexity.