Microbial Whole Genome Sequencing

Comprehensive whole-genome sequencing of microorganisms to characterize genetic variation, genome structure, and functional potential.

Microbial Whole Genome Sequencing (mWGS) is an advanced and efficient method for analyzing entire microbial genomes, including bacteria and viruses, to generate accurate reference genomes, identify microbes, and support various comparative genomic studies. Unlike traditional PCR, mWGS eliminates labor-intensive cloning and mapping, making it quicker and more cost-effective.

At Novogene, our high-throughput sequencing technology enables faster microbial identification, supporting a wide range of research applications.

Why Choose Novogene for mWGS? What we offer?

Fast Turnaround

Receive high-quality sequencing data in as little as two weeks.

Comprehensive Analysis

Detects mutations (SNP, InDel, CNV, SV) and studies evolution and population traits.

Localized Support

Regional technical teams provide personalized assistance for your unique needs.

Customizable Solutions

Expertly designed projects for complex samples and challenging studies.

Professional Services

From material selection to data analysis, every step is designed for precise results.

High-Quality Library Preparation

Size selection ensures optimized insert size and reliable data quality.

Applications of Microbial Whole Genome Sequencing

Unlock deep insights into microbial genetics and evolution with whole genome sequencing applications.

Detect Genetic Variations

Accurately identify genome differences by comparing sequences to reference genomes.

Interpret Genetic Traits

Link genetic variations to phenotypic traits such as virulence or benignity.

Trace Microbial Evolution

Study SNP patterns to uncover microbial origins, spread, and diversification.

Identify Novel Species

Leverage genetic data to define and classify previously unknown microbial species cultured in labs.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Demo Results

1/1

Mutation Spectrum

Compare mutation type proportions across samples to review overall sequence variation patterns.

1/1

COG Functional Classification

Summarise predicted gene functions by COG category to support functional genome interpretation.

1/1

Coding Variant Effects

Summarise coding-region variant effects, including synonymous, non-synonymous and stop-gain or stop-loss changes.

More Services

Microbial De novo Sequencing

Shotgun Metagenomic Sequencing

Amplicon Sequencing

Metatranscriptome Sequencing

Prokaryotic RNA Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Microbial Whole Genome Sequencing

Comprehensive whole-genome sequencing of microorganisms to characterize genetic variation, genome structure, and functional potential.

At Novogene, our high-throughput sequencing technology enables faster microbial identification, supporting a wide range of research applications.

Why Choose Novogene for mWGS? What we offer?

Fast Turnaround

Receive high-quality sequencing data in as little as two weeks.

Comprehensive Analysis

Detects mutations (SNP, InDel, CNV, SV) and studies evolution and population traits.

Localized Support

Regional technical teams provide personalized assistance for your unique needs.

Customizable Solutions

Expertly designed projects for complex samples and challenging studies.

Professional Services

From material selection to data analysis, every step is designed for precise results.

High-Quality Library Preparation

Size selection ensures optimized insert size and reliable data quality.

Applications of Microbial Whole Genome Sequencing

Unlock deep insights into microbial genetics and evolution with whole genome sequencing applications.

Detect Genetic Variations

Accurately identify genome differences by comparing sequences to reference genomes.

Interpret Genetic Traits

Link genetic variations to phenotypic traits such as virulence or benignity.

Trace Microbial Evolution

Study SNP patterns to uncover microbial origins, spread, and diversification.

Identify Novel Species

Leverage genetic data to define and classify previously unknown microbial species cultured in labs.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount (Qubit)	Volume	Concentration	Purity
Microbial whole genome library	Genomic DNA	≥ 100 ng	≥ 20 μL	≥ 5 ng/μL	A260/280=1.8-2.0; no degradation, no contamination
Microbial whole genome library (PCR-free)	Genomic DNA	≥ 1.2 μg	≥ 20 μL	≥ 50 ng/μL	A260/280=1.8-2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq Platforms
Read Length	Paired-end 150 bp
Recommended Sequencing Depth	≥ 100x for bacterial genomes
Recommended Sequencing Depth	≥ 100x for fungal genomes
Standard Data Analysis	Data quality control: filtering reads containing adapter or with low quality Alignment with the reference genome, statistics of sequencing depth and coverage SNP/InDel calling, annotation and statistics CNV calling, annotation and statistics SV calling, annotation and statistics

Project Workflow

Demo Results

1/1

Mutation Spectrum

Compare mutation type proportions across samples to review overall sequence variation patterns.

1/1

COG Functional Classification

Summarise predicted gene functions by COG category to support functional genome interpretation.

1/1

Coding Variant Effects

Summarise coding-region variant effects, including synonymous, non-synonymous and stop-gain or stop-loss changes.

More Services

Microbial De novo Sequencing

Shotgun Metagenomic Sequencing

Amplicon Sequencing

Metatranscriptome Sequencing

Prokaryotic RNA Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.