Untargeted Metabolomics

Untargeted metabolomics enables broad, unbiased profiling of metabolites across biological systems, making it ideal for large-scale metabolic screening and discovery.

By combining high-resolution LC-MS with a rigorously curated, in-house standard library, Novogene’s Untargeted Metabolomics Plus and Pro services deliver both extensive metabolite coverage and high-confidence identification. This powerful platform ensures accurate detection of diverse metabolites while seamlessly integrating with other omics technologies to reveal meaningful biological insights.

Why Choose Novogene for Your Metabolomics Needs?

Extensive, Curated Database

Benefit from a robust metabolite library built on 20,000+ reference standards and an expanded spectral database of over 300,000 metabolites.

High-Confidence Metabolite Identification

The Pro and Plus services provide reliable level 1 identification and comprehensive compound detection to support high-impact biological discovery.

Enhanced Metabolome Coverage (Pro Only)

Combined C18 and HILIC separation ensures broad coverage of both polar and non-polar metabolites in a single study.

Comprehensive Metabolite Information

Each metabolite is annotated with more than 27 standardized data fields, including CAS and classification information.

Applications of Untargeted Metabolomics in Medical and Agricultural Research

Explore the power of metabolomics with Novogene’s services, built to advance a broad spectrum of research aims.

Precision Medicine

Identify metabolic biomarkers for disease diagnosis, prognosis, and personalized treatment guidance.

Drug Discovery and Development

Clarify drug mechanisms and evaluate safety and toxicity through metabolic pathway analysis.

Disease Mechanism Studies

Reveal dysregulated metabolic pathways underlying cancer, metabolic disorders, and neurodegenerative diseases.

Health, Development, and Aging

Characterize metabolic changes associated with development, aging, lifestyle, and overall health status.

Agriculture and Food Science

Analyze metabolic responses to environmental stresses such as heat, drought, and salinity.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

Metabolomics research follows a streamlined workflow — from sample preparation and metabolite extraction to LC-MS analysis, database-driven identification, and bioinformatic interpretation — delivering a clear view of metabolic activity.

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Demo Results

1/1

Data Quality Control

Quality control ensures timely issue resolution and reliable data.

1/1

Metabolite Pathway and Classification Annotation

Identified metabolites are annotated by pathway and classification to reveal their functions and categories.

1/1

Differential Metabolite Screening

Visualize the distribution of differential metabolites.

1/1

KEGG Analysis

Identify key metabolic and signaling pathways of differential metabolites.

1/1

GSEA Analysis

Identify biologically relevant metabolite sets linked to specific conditions.

1/1

Multi-omics Analysis

Correlation between two omics data.

Frequently Asked Questions

Why does untargeted metabolomics use both positive and negative ion modes?

A: Different metabolites have different ionization properties—some tend to carry positive charges, while others tend to carry negative charges. To detect a broader range of metabolites, analyses are typically performed separately in positive (pos) and negative (neg) ion modes. Some amphoteric compounds may be detected in both modes. By default, results are analyzed separately for each mode; however, if a combined bioinformatics analysis of positive and negative ion results is required, it must be specified in advance.

Can quantitative values of different substances in the same untargeted metabolomics sample be compared?

Possible Reasons for Missing Target Compounds in Untargeted Metabolomics Identification?

What is the recommended number of samples for untargeted metabolomics?

More Services

Quantitative Proteomics

Olink Proteomics

Shotgun Metagenomic Sequencing

Amplicon Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Untargeted Metabolomics

Untargeted metabolomics enables broad, unbiased profiling of metabolites across biological systems, making it ideal for large-scale metabolic screening and discovery.

Why Choose Novogene for Your Metabolomics Needs?

Extensive, Curated Database

Benefit from a robust metabolite library built on 20,000+ reference standards and an expanded spectral database of over 300,000 metabolites.

High-Confidence Metabolite Identification

The Pro and Plus services provide reliable level 1 identification and comprehensive compound detection to support high-impact biological discovery.

Enhanced Metabolome Coverage (Pro Only)

Combined C18 and HILIC separation ensures broad coverage of both polar and non-polar metabolites in a single study.

Comprehensive Metabolite Information

Each metabolite is annotated with more than 27 standardized data fields, including CAS and classification information.

Applications of Untargeted Metabolomics in Medical and Agricultural Research

Explore the power of metabolomics with Novogene’s services, built to advance a broad spectrum of research aims.

Precision Medicine

Identify metabolic biomarkers for disease diagnosis, prognosis, and personalized treatment guidance.

Drug Discovery and Development

Clarify drug mechanisms and evaluate safety and toxicity through metabolic pathway analysis.

Disease Mechanism Studies

Reveal dysregulated metabolic pathways underlying cancer, metabolic disorders, and neurodegenerative diseases.

Health, Development, and Aging

Characterize metabolic changes associated with development, aging, lifestyle, and overall health status.

Agriculture and Food Science

Analyze metabolic responses to environmental stresses such as heat, drought, and salinity.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Sample Amount
Human & Animal Tissue	40mg
Plant Tissue	40mg
Plasma/ Serum	40μL
Urine	40μL
Saliva	200μL
Feces/Intestinal contents	40mg

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Q Exactive™ HF-X Platform Performance Parameters
Resolution	240,000 @ m/z 200
Mass Range	50–6,000 m/z
Scan Rate	Up to 40 Hz
Mass Accuracy	Internal standard <1 ppm RMS; external standard <3 ppm RMS
Sensitivity	Full scan MS: 100 fg on-column with a signal-to-noise ratio (S/N) of 150:1 SIM: 50 fg on-column with a signal-to-noise ratio (S/N) of 150:1
Dynamic Range	> 5,000:1

Project Workflow

At Novogene, every step is executed with strict quality control and technical precision, ensuring reliable, reproducible data that you can trust to drive meaningful biological insights.

Demo Results

1/1

Data Quality Control

Quality control ensures timely issue resolution and reliable data.

1/1

Metabolite Pathway and Classification Annotation

Identified metabolites are annotated by pathway and classification to reveal their functions and categories.

1/1

Differential Metabolite Screening

Visualize the distribution of differential metabolites.

1/1

KEGG Analysis

Identify key metabolic and signaling pathways of differential metabolites.

1/1

GSEA Analysis

Identify biologically relevant metabolite sets linked to specific conditions.

1/1

Multi-omics Analysis

Correlation between two omics data.

Frequently Asked Questions

Why does untargeted metabolomics use both positive and negative ion modes?

Can quantitative values of different substances in the same untargeted metabolomics sample be compared?

Possible Reasons for Missing Target Compounds in Untargeted Metabolomics Identification?

What is the recommended number of samples for untargeted metabolomics?

More Services

Quantitative Proteomics

Olink Proteomics

Shotgun Metagenomic Sequencing

Amplicon Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.