Novogene AMEA
  • Novogene AMEA
  • Genomics
    • Human Whole Genome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Microbial Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial De novo Sequencing
    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing
    • Whole Exome Sequencing
    Transcriptomics
    • mRNA Sequencing
    • Total RNA Sequencing
    • Full-Length Transcriptome Sequencing
    • Whole Transcriptome Sequencing
    • Small RNA Sequencing
    • Circular RNA Sequencing
    • Metatranscriptome Sequencing
    • Prokaryotic RNA Sequencing
    Single Cell & Spatial Omics
    • Single Cell Gene Expression
    • Single Cell Immune Profiling Sequencing
    • Single Cell Long Read Transcriptome
    • Visium HD Spatial Gene Expression
    • Stereo-Seq Spatial Gene Expression
    • Xenium In Situ Spatial Transcriptome
    Epigenomics
    • Whole Genome Bisulfite Sequencing (WGBS)
    • Directed DNA Methylation Sequencing (DM-Seq) NEW
    • Reduced Representation Bisulfite Sequencing (RRBS)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on PacBio Sequencer
    Proteomics and Metabolomics
    • Olink Proteomics
    • Quantitative Proteomics
    • Untargeted Metabolomics
  • PromotionsPromotions
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    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
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    • Sample Guidelines
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • Our Locations
    • News
    • Careers
  • Contact UsContact Us

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Whole Genome SequencingDe novo SequencingAmplicon SequencingShotgun Metagenomic SequencingDirected DNA Methylation Sequencing (DM-Seq)mRNA SequencingSingle Cell Gene ExpressionVisium HD Spatial Gene ExpressionXenium In Situ Spatial TranscriptomeOlink ProteomicsUntargeted Metabolomics
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NovoMagic Bioinformatics Analysis ToolCustomer Service SystemFalcon Intelligent Delivery Platform
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Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
Novogene AMEA
  • Novogene AMEA
  • Genomics
    • Human Whole Genome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Microbial Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial De novo Sequencing
    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing
    • Whole Exome Sequencing
    Transcriptomics
    • mRNA Sequencing
    • Total RNA Sequencing
    • Full-Length Transcriptome Sequencing
    • Whole Transcriptome Sequencing
    • Small RNA Sequencing
    • Circular RNA Sequencing
    • Metatranscriptome Sequencing
    • Prokaryotic RNA Sequencing
    Single Cell & Spatial Omics
    • Single Cell Gene Expression
    • Single Cell Immune Profiling Sequencing
    • Single Cell Long Read Transcriptome
    • Visium HD Spatial Gene Expression
    • Stereo-Seq Spatial Gene Expression
    • Xenium In Situ Spatial Transcriptome
    Epigenomics
    • Whole Genome Bisulfite Sequencing (WGBS)
    • Directed DNA Methylation Sequencing (DM-Seq) NEW
    • Reduced Representation Bisulfite Sequencing (RRBS)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on PacBio Sequencer
    Proteomics and Metabolomics
    • Olink Proteomics
    • Quantitative Proteomics
    • Untargeted Metabolomics
  • PromotionsPromotions
    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Brochures
    • Case Studies
    • Webinar
    • Blog
    • Sample Guidelines
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • Our Locations
    • News
    • Careers
  • Contact UsContact Us

ServicesServices menu

SupportSupport menu

CompanyCompany menu

Services
Whole Genome SequencingDe novo SequencingAmplicon SequencingShotgun Metagenomic SequencingDirected DNA Methylation Sequencing (DM-Seq)mRNA SequencingSingle Cell Gene ExpressionVisium HD Spatial Gene ExpressionXenium In Situ Spatial TranscriptomeOlink ProteomicsUntargeted Metabolomics
Support
NovoMagic Bioinformatics Analysis ToolCustomer Service SystemFalcon Intelligent Delivery Platform
Company
About UsOur LocationsOur PlatformsNewsCareersContact Us
LinkedInLinkedIn hoverYouTubeYouTube hoverXX hover
Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
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Microbial De novo Sequencing

De novo assembly of microbial genomes to enable accurate characterization of novel strains and genomic features.
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(Microbial De novo Sequencing)
Request a Quote
(Microbial De novo Sequencing)
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

De novo sequencing can sequence the genome of a species without any reference genome information, splice and assemble it by bioinformatics analysis methods, and obtain the genome sequence map of the species, to promote the follow-up research of the species. It offers reference genome assembly for rarely studied species. Using de novo sequencing to obtain the genomic information of microbes provides a fresh start for exploring the genetic structure and functions, studying the evolutionary origin of microbial populations, as well as developing potential applications of these abundant microbes in medicine, disease, agriculture, and the environment.


Novogene offers de novo sequencing services using both PacBio/Nanopore and Illumina platforms. We provide multifaceted sequencing services including genome survey, draft map, complete map, and fine map tailored to different research needs. For each project, our scientists will design the best sequencing strategy utilizing an optimal combination of short reads and long-range sequencing information to achieve the most comprehensive de novo assembly results for your genome of interest.

Benefits

Highly experienced
Highly experienced

We have completed numerous microbial De novo sequencing projects with 21 publications in top-tier journals.

Highly experienced
Highly experienced

We have completed numerous microbial De novo sequencing projects with 21 publications in top-tier journals.

Largest sequencing capacity
Largest sequencing capacity

We offer world-leading sequencing capacity, delivering high-quality data with fast turnaround and cost efficiency.

Largest sequencing capacity
Largest sequencing capacity

We offer world-leading sequencing capacity, delivering high-quality data with fast turnaround and cost efficiency.

In-depth data mining
In-depth data mining

We provide complete solutions for analyzing individual variation and population evolution to address key biological questions.

In-depth data mining
In-depth data mining

We provide complete solutions for analyzing individual variation and population evolution to address key biological questions.

Accuracy
Accuracy

Creates accurate reference sequences, even for complicated or polyploid genomes.

Accuracy
Accuracy

Creates accurate reference sequences, even for complicated or polyploid genomes.

Benefits

Highly experienced
Highly experienced

We have completed numerous microbial De novo sequencing projects with 21 publications in top-tier journals.

Highly experienced
Highly experienced

We have completed numerous microbial De novo sequencing projects with 21 publications in top-tier journals.

Largest sequencing capacity
Largest sequencing capacity

We offer world-leading sequencing capacity, delivering high-quality data with fast turnaround and cost efficiency.

Largest sequencing capacity
Largest sequencing capacity

We offer world-leading sequencing capacity, delivering high-quality data with fast turnaround and cost efficiency.

In-depth data mining
In-depth data mining

We provide complete solutions for analyzing individual variation and population evolution to address key biological questions.

In-depth data mining
In-depth data mining

We provide complete solutions for analyzing individual variation and population evolution to address key biological questions.

Accuracy
Accuracy

Creates accurate reference sequences, even for complicated or polyploid genomes.

Accuracy
Accuracy

Creates accurate reference sequences, even for complicated or polyploid genomes.

Applications

Virulence Research

Aims to elucidate the specific molecular mechanisms by which a pathogen invades a host and causes disease.

Virulence Research

Aims to elucidate the specific molecular mechanisms by which a pathogen invades a host and causes disease.

Drug Resistance Mechanism

Focuses on how microbes evolve mechanisms, such as genetic mutations or acquiring resistance genes, to evade the effects of antimicrobial drugs.

Drug Resistance Mechanism

Focuses on how microbes evolve mechanisms, such as genetic mutations or acquiring resistance genes, to evade the effects of antimicrobial drugs.

Molecular Markers

Involves identifying specific DNA sequences or proteins to rapidly and accurately identify the species or strain of a pathogen.

Molecular Markers

Involves identifying specific DNA sequences or proteins to rapidly and accurately identify the species or strain of a pathogen.

Vaccine Development

The process of designing safe antigenic components or attenuated pathogens to stimulate a protective immune response for preventing infection.

Vaccine Development

The process of designing safe antigenic components or attenuated pathogens to stimulate a protective immune response for preventing infection.

Applications

Virulence Research

Aims to elucidate the specific molecular mechanisms by which a pathogen invades a host and causes disease.

Virulence Research

Aims to elucidate the specific molecular mechanisms by which a pathogen invades a host and causes disease.

Drug Resistance Mechanism

Focuses on how microbes evolve mechanisms, such as genetic mutations or acquiring resistance genes, to evade the effects of antimicrobial drugs.

Drug Resistance Mechanism

Focuses on how microbes evolve mechanisms, such as genetic mutations or acquiring resistance genes, to evade the effects of antimicrobial drugs.

Molecular Markers

Involves identifying specific DNA sequences or proteins to rapidly and accurately identify the species or strain of a pathogen.

Molecular Markers

Involves identifying specific DNA sequences or proteins to rapidly and accurately identify the species or strain of a pathogen.

Vaccine Development

The process of designing safe antigenic components or attenuated pathogens to stimulate a protective immune response for preventing infection.

Vaccine Development

The process of designing safe antigenic components or attenuated pathogens to stimulate a protective immune response for preventing infection.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Demo Results

Image
Image
1/1
Whole-Genome Circular Map

This Circos plot displays the assembled genome sequence of a sample, along with its predicted coding genes, and additional relevant results if non-coding RNA analysis and gene function annotation were performed.

Image
Image
1/1
SV Statistics and Annotation

This pairwise map shows whole-genome structural variations (insertions, deletions, inversions, translocations, Trans+Inver) identified via genome alignments and adjacent alignment block distances.

Image
Image
1/1
Whole-Genome Synteny Analysis

This visualization shows synteny between sample and reference genomes, reflecting evolutionary distance and genetic relationships.

Demo Results

Image
Image
1/1
Whole-Genome Circular Map

This Circos plot displays the assembled genome sequence of a sample, along with its predicted coding genes, and additional relevant results if non-coding RNA analysis and gene function annotation were performed.

Image
Image
1/1
SV Statistics and Annotation

This pairwise map shows whole-genome structural variations (insertions, deletions, inversions, translocations, Trans+Inver) identified via genome alignments and adjacent alignment block distances.

Image
Image
1/1
Whole-Genome Synteny Analysis

This visualization shows synteny between sample and reference genomes, reflecting evolutionary distance and genetic relationships.

Image
Image
1/1
Whole-Genome Circular Map

This Circos plot displays the assembled genome sequence of a sample, along with its predicted coding genes, and additional relevant results if non-coding RNA analysis and gene function annotation were performed.

Image
Image
1/1
SV Statistics and Annotation

This pairwise map shows whole-genome structural variations (insertions, deletions, inversions, translocations, Trans+Inver) identified via genome alignments and adjacent alignment block distances.

Image
Image
1/1
Whole-Genome Synteny Analysis

This visualization shows synteny between sample and reference genomes, reflecting evolutionary distance and genetic relationships.

More Services

Plant and Animal de novo Sequencing
(Plant and Animal de novo Sequencing)
Plant and Animal de novo Sequencing
(Plant and Animal de novo Sequencing)
Microbial Whole Genome Sequencing
(Microbial Whole Genome Sequencing)
Microbial Whole Genome Sequencing
(Microbial Whole Genome Sequencing)
Whole Transcriptome Sequencing
(Whole Transcriptome Sequencing)
Whole Transcriptome Sequencing
(Whole Transcriptome Sequencing)

More Services

Plant and Animal de novo Sequencing
(Plant and Animal de novo Sequencing)
Plant and Animal de novo Sequencing
(Plant and Animal de novo Sequencing)
Microbial Whole Genome Sequencing
(Microbial Whole Genome Sequencing)
Microbial Whole Genome Sequencing
(Microbial Whole Genome Sequencing)
Whole Transcriptome Sequencing
(Whole Transcriptome Sequencing)
Whole Transcriptome Sequencing
(Whole Transcriptome Sequencing)
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Microbial De novo Sequencing

De novo assembly of microbial genomes to enable accurate characterization of novel strains and genomic features.
Request a Quote
(Microbial De novo Sequencing)
Request a Quote
(Microbial De novo Sequencing)
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

De novo sequencing can sequence the genome of a species without any reference genome information, splice and assemble it by bioinformatics analysis methods, and obtain the genome sequence map of the species, to promote the follow-up research of the species. It offers reference genome assembly for rarely studied species. Using de novo sequencing to obtain the genomic information of microbes provides a fresh start for exploring the genetic structure and functions, studying the evolutionary origin of microbial populations, as well as developing potential applications of these abundant microbes in medicine, disease, agriculture, and the environment.


Novogene offers de novo sequencing services using both PacBio/Nanopore and Illumina platforms. We provide multifaceted sequencing services including genome survey, draft map, complete map, and fine map tailored to different research needs. For each project, our scientists will design the best sequencing strategy utilizing an optimal combination of short reads and long-range sequencing information to achieve the most comprehensive de novo assembly results for your genome of interest.

Benefits

Highly experienced
Highly experienced

We have completed numerous microbial De novo sequencing projects with 21 publications in top-tier journals.

Highly experienced
Highly experienced

We have completed numerous microbial De novo sequencing projects with 21 publications in top-tier journals.

Largest sequencing capacity
Largest sequencing capacity

We offer world-leading sequencing capacity, delivering high-quality data with fast turnaround and cost efficiency.

Largest sequencing capacity
Largest sequencing capacity

We offer world-leading sequencing capacity, delivering high-quality data with fast turnaround and cost efficiency.

In-depth data mining
In-depth data mining

We provide complete solutions for analyzing individual variation and population evolution to address key biological questions.

In-depth data mining
In-depth data mining

We provide complete solutions for analyzing individual variation and population evolution to address key biological questions.

Accuracy
Accuracy

Creates accurate reference sequences, even for complicated or polyploid genomes.

Accuracy
Accuracy

Creates accurate reference sequences, even for complicated or polyploid genomes.

Benefits

Highly experienced
Highly experienced

We have completed numerous microbial De novo sequencing projects with 21 publications in top-tier journals.

Highly experienced
Highly experienced

We have completed numerous microbial De novo sequencing projects with 21 publications in top-tier journals.

Largest sequencing capacity
Largest sequencing capacity

We offer world-leading sequencing capacity, delivering high-quality data with fast turnaround and cost efficiency.

Largest sequencing capacity
Largest sequencing capacity

We offer world-leading sequencing capacity, delivering high-quality data with fast turnaround and cost efficiency.

In-depth data mining
In-depth data mining

We provide complete solutions for analyzing individual variation and population evolution to address key biological questions.

In-depth data mining
In-depth data mining

We provide complete solutions for analyzing individual variation and population evolution to address key biological questions.

Accuracy
Accuracy

Creates accurate reference sequences, even for complicated or polyploid genomes.

Accuracy
Accuracy

Creates accurate reference sequences, even for complicated or polyploid genomes.

Applications

Virulence Research

Aims to elucidate the specific molecular mechanisms by which a pathogen invades a host and causes disease.

Virulence Research

Aims to elucidate the specific molecular mechanisms by which a pathogen invades a host and causes disease.

Drug Resistance Mechanism

Focuses on how microbes evolve mechanisms, such as genetic mutations or acquiring resistance genes, to evade the effects of antimicrobial drugs.

Drug Resistance Mechanism

Focuses on how microbes evolve mechanisms, such as genetic mutations or acquiring resistance genes, to evade the effects of antimicrobial drugs.

Molecular Markers

Involves identifying specific DNA sequences or proteins to rapidly and accurately identify the species or strain of a pathogen.

Molecular Markers

Involves identifying specific DNA sequences or proteins to rapidly and accurately identify the species or strain of a pathogen.

Vaccine Development

The process of designing safe antigenic components or attenuated pathogens to stimulate a protective immune response for preventing infection.

Vaccine Development

The process of designing safe antigenic components or attenuated pathogens to stimulate a protective immune response for preventing infection.

Applications

Virulence Research

Aims to elucidate the specific molecular mechanisms by which a pathogen invades a host and causes disease.

Virulence Research

Aims to elucidate the specific molecular mechanisms by which a pathogen invades a host and causes disease.

Drug Resistance Mechanism

Focuses on how microbes evolve mechanisms, such as genetic mutations or acquiring resistance genes, to evade the effects of antimicrobial drugs.

Drug Resistance Mechanism

Focuses on how microbes evolve mechanisms, such as genetic mutations or acquiring resistance genes, to evade the effects of antimicrobial drugs.

Molecular Markers

Involves identifying specific DNA sequences or proteins to rapidly and accurately identify the species or strain of a pathogen.

Molecular Markers

Involves identifying specific DNA sequences or proteins to rapidly and accurately identify the species or strain of a pathogen.

Vaccine Development

The process of designing safe antigenic components or attenuated pathogens to stimulate a protective immune response for preventing infection.

Vaccine Development

The process of designing safe antigenic components or attenuated pathogens to stimulate a protective immune response for preventing infection.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Revio System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes
≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft Map Standard Analysis
· Data quality control
· Genome preliminarily assembly
· Genome component analysis: gene structure prediction, repetitive sequences, non-coding RNAs
· Gene function annotation
Bacteria Complete Map Standard Analysis
· Data quality control
· Genome assembly · Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
· Gene function annotation
-GO annotation,
-KEGG annotation,
-COG  annotation
-NR annotation,
-TCDB annotation
-Pfam annotation
Swiss-Prot annotation
· Genome cycle graph
Fungus Fine Map Standard Analysis
· Data quality control · · Genome advanced assembly
· Genome survey
· Genome component analysis
· Repeat annotation
· Coding gene annotation
· ncRNA annotation
·Gene function annotation
-GO annotation,
-KEGG annotation,
-NR annotation,
-KOG annotation
-Pfam annotation
-Swiss-Prot annotation.

Project Workflow

Project Workflow

Demo Results

Image
Image
1/1
Whole-Genome Circular Map

This Circos plot displays the assembled genome sequence of a sample, along with its predicted coding genes, and additional relevant results if non-coding RNA analysis and gene function annotation were performed.

Image
Image
1/1
SV Statistics and Annotation

This pairwise map shows whole-genome structural variations (insertions, deletions, inversions, translocations, Trans+Inver) identified via genome alignments and adjacent alignment block distances.

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Image
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Whole-Genome Synteny Analysis

This visualization shows synteny between sample and reference genomes, reflecting evolutionary distance and genetic relationships.

Demo Results

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Image
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Whole-Genome Circular Map

This Circos plot displays the assembled genome sequence of a sample, along with its predicted coding genes, and additional relevant results if non-coding RNA analysis and gene function annotation were performed.

Image
Image
1/1
SV Statistics and Annotation

This pairwise map shows whole-genome structural variations (insertions, deletions, inversions, translocations, Trans+Inver) identified via genome alignments and adjacent alignment block distances.

Image
Image
1/1
Whole-Genome Synteny Analysis

This visualization shows synteny between sample and reference genomes, reflecting evolutionary distance and genetic relationships.

Image
Image
1/1
Whole-Genome Circular Map

This Circos plot displays the assembled genome sequence of a sample, along with its predicted coding genes, and additional relevant results if non-coding RNA analysis and gene function annotation were performed.

Image
Image
1/1
SV Statistics and Annotation

This pairwise map shows whole-genome structural variations (insertions, deletions, inversions, translocations, Trans+Inver) identified via genome alignments and adjacent alignment block distances.

Image
Image
1/1
Whole-Genome Synteny Analysis

This visualization shows synteny between sample and reference genomes, reflecting evolutionary distance and genetic relationships.

More Services

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Whole Transcriptome Sequencing
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More Services

Plant and Animal de novo Sequencing
(Plant and Animal de novo Sequencing)
Plant and Animal de novo Sequencing
(Plant and Animal de novo Sequencing)
Microbial Whole Genome Sequencing
(Microbial Whole Genome Sequencing)
Microbial Whole Genome Sequencing
(Microbial Whole Genome Sequencing)
Whole Transcriptome Sequencing
(Whole Transcriptome Sequencing)
Whole Transcriptome Sequencing
(Whole Transcriptome Sequencing)
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