Total RNA Sequencing

Comprehensive transcriptome profiling of coding and noncoding RNAs to reveal gene expression patterns and transcriptomic complexity.

Total RNA-seq captures the entire RNA landscape beyond mRNA — including regulatory molecules like lncRNA — to deliver a holistic view of gene expression and regulation. It empowers researchers to discover novel transcripts, investigate alternative splicing, analyze expression changes across conditions, and explore functional mechanisms in fields such as disease research, drug response, and developmental biology.

Novogene's Total RNA solution combines an optimized library preparation protocol for superior sensitivity with a complete bioinformatics pipeline. From precise strand-origin identification to lncRNA-mRNA regulatory network analysis, we deliver actionable insights through a streamlined, end-to-end workflow.

Why Choose Novogene for Total RNA Sequencing?

High-Performance Sequencing

Achieve high-throughput, high-accuracy data (Q30 ≥ 85%) with minimal starting material.

Proven Service Track Record

Benefit from our extensive experience, supporting thousands of projects and high-impact publications.

Comprehensive Total RNA Analysis

Access all-inclusive solutions for identification, quantification, and differential expression analysis.

Publication-Ready Bioinformatics Support

Obtain customized, publication-quality results backed by our expert bioinformatics team.

Applications of Total RNA-Seq

Total RNA-seq unlocks a deeper understanding of the transcriptome by capturing both coding and noncoding RNA, enabling broad research and clinical discovery.

In-Depth Exploration

Profile both known and novel transcripts, identify expression changes, and detect sequence variations across the whole transcriptome.

Uncover Novel Insights

Discover biomarkers for disease diagnosis, classification, and therapeutic response through comprehensive RNA expression and lncRNA analysis.

Target Prediction

Predict RNA–RNA and RNA–gene interactions to reveal regulatory targets and functional pathways.

Comprehensive Analysis

Investigate regulatory relationships between coding and noncoding RNAs to understand gene expression networks and molecular mechanisms.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

The workflow of the Total RNA-seq starts with sample preparation and quality control. The ribosomal RNA (rRNA) is depleted for target transcript enrichment. The fragmented RNA undergoes reverse transcription into cDNA. Strand-specific libraries (also known as stranded libraries, directional libraries) are prepared, and the sequencing is performed using a paired-end 150bp strategy on the Illumina platform. The downstream processing follows the well-established Novogene pipeline, which guarantees the highest quality results. Customized bioinformatics solutions are available upon request.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Demo Results

1/1

Coding Potential Prediction

Coding potential analysis is a key step to distinguish lncRNAs from other transcripts. We integrate predictions from multiple established algorithms — including CPC, CNCI, and Pfam — using a consensus approach for reliable classification.

1/1

Structural Comparison: lncRNA vs. mRNA

We compare key structural features — such as transcript length, exon count, and ORF length — to reveal the characteristic differences between lncRNAs and mRNAs.

1/1

Expression Level Distribution

This chart displays expression levels across samples, with the x-axis showing sample names and the y-axis representing log₁₀(FPKM+1) values.

1/1

Volcano Plot of Differentially Expressed Genes (DEGs)

The volcano plot visualizes differential expression, where the x-axis indicates log₂ fold change and the y-axis represents statistical significance (--log₁₀ p-value).

1/1

Clustering Heatmap of DEGs

Differentially expressed genes are clustered via hierarchical clustering based on their expression patterns (row-normalized). The heatmap groups genes or samples with similar profiles for intuitive visualization.

Demo Results

1/1

Coding Potential Prediction

1/1

Structural Comparison: lncRNA vs. mRNA

We compare key structural features — such as transcript length, exon count, and ORF length — to reveal the characteristic differences between lncRNAs and mRNAs.

1/1

Expression Level Distribution

This chart displays expression levels across samples, with the x-axis showing sample names and the y-axis representing log₁₀(FPKM+1) values.

1/1

Volcano Plot of Differentially Expressed Genes (DEGs)

The volcano plot visualizes differential expression, where the x-axis indicates log₂ fold change and the y-axis represents statistical significance (--log₁₀ p-value).

1/1

Clustering Heatmap of DEGs

More Services

Small RNA Sequencing

CircRNA Sequencing

mRNA Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Total RNA Sequencing

Comprehensive transcriptome profiling of coding and noncoding RNAs to reveal gene expression patterns and transcriptomic complexity.

Why Choose Novogene for Total RNA Sequencing?

High-Performance Sequencing

Achieve high-throughput, high-accuracy data (Q30 ≥ 85%) with minimal starting material.

Proven Service Track Record

Benefit from our extensive experience, supporting thousands of projects and high-impact publications.

Comprehensive Total RNA Analysis

Access all-inclusive solutions for identification, quantification, and differential expression analysis.

Publication-Ready Bioinformatics Support

Obtain customized, publication-quality results backed by our expert bioinformatics team.

Applications of Total RNA-Seq

Total RNA-seq unlocks a deeper understanding of the transcriptome by capturing both coding and noncoding RNA, enabling broad research and clinical discovery.

In-Depth Exploration

Profile both known and novel transcripts, identify expression changes, and detect sequence variations across the whole transcriptome.

Uncover Novel Insights

Discover biomarkers for disease diagnosis, classification, and therapeutic response through comprehensive RNA expression and lncRNA analysis.

Target Prediction

Predict RNA–RNA and RNA–gene interactions to reveal regulatory targets and functional pathways.

Comprehensive Analysis

Investigate regulatory relationships between coding and noncoding RNAs to understand gene expression networks and molecular mechanisms.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity (NanoDrop)
lncRNA Library	Total RNA	≥ 300 ng	≥ 5.5, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no genomic contamination
Exosomal lncRNA Library	Exosomal RNA	≥ 10 ng	Fragments between 80-200 nt, no peaks > 2000 nt, FU*>10, with flat baseline
Dual RNA library	Total RNA	≥ 400 ng	≥ 6.5, with flat base line

*FU: Fluorescent unit

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Recommended Sequencing Depth	≥ 40 million read pairs per sample for species with reference genome
Standard Data Analysis	•Data quality control •Structural analysis (alternative splicing & variation calling) •lncRNA identification & annotation •Expression quantification & differential expression profiling •Functional enrichment analysis •Protein-protein interaction (PPI) analysis •lncRNA target gene prediction

Project Workflow

Demo Results

1/1

Coding Potential Prediction

1/1

Structural Comparison: lncRNA vs. mRNA

We compare key structural features — such as transcript length, exon count, and ORF length — to reveal the characteristic differences between lncRNAs and mRNAs.

1/1

Expression Level Distribution

This chart displays expression levels across samples, with the x-axis showing sample names and the y-axis representing log₁₀(FPKM+1) values.

1/1

Volcano Plot of Differentially Expressed Genes (DEGs)

The volcano plot visualizes differential expression, where the x-axis indicates log₂ fold change and the y-axis represents statistical significance (--log₁₀ p-value).

1/1

Clustering Heatmap of DEGs

Demo Results

1/1

Coding Potential Prediction

1/1

Structural Comparison: lncRNA vs. mRNA

We compare key structural features — such as transcript length, exon count, and ORF length — to reveal the characteristic differences between lncRNAs and mRNAs.

1/1

Expression Level Distribution

This chart displays expression levels across samples, with the x-axis showing sample names and the y-axis representing log₁₀(FPKM+1) values.

1/1

Volcano Plot of Differentially Expressed Genes (DEGs)

The volcano plot visualizes differential expression, where the x-axis indicates log₂ fold change and the y-axis represents statistical significance (--log₁₀ p-value).

1/1

Clustering Heatmap of DEGs

More Services

Small RNA Sequencing

CircRNA Sequencing

mRNA Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.