Single Cell Gene Expression

High-resolution single-cell transcriptome profiling to reveal cellular heterogeneity and gene expression dynamics across complex populations.

Single-cell RNA sequencing (scRNA-seq) enables the detection of cell-to-cell differences and cellular heterogeneity that are completely obscured by bulk RNA-seq. It provides high-resolution insights into gene expression at the individual cell level. With broad applications across biology and medicine, single-cell sequencing offers unprecedented understanding of cellular behavior, function, and regulation.

Powered by the 10x Genomics® Chromium™ X technology, the Illumina® NovaSeq™ X Plus platform, Novogene provides end-to-end single-cell RNA sequencing (scRNA-seq) solutions — from sample processing and library preparation to sequencing and bioinformatic analysis. Delivered with deep technical expertise and a customer-focused approach, our solutions enable high-resolution exploration of the transcriptome and immune profiling at the single-cell level.

Why Choose Novogene for Single Cell Gene Expression Sequencing?

End-to-End Service

Seamless scRNA-seq workflow from sample processing to bioinformatics with publication-ready outputs

Proven Expertise

High-quality results with industry-leading turnaround and extensive experience across challenging sample types

Enhanced Sample Processing

Flexible processing including nuclei extraction and specialized frozen tissue workflows for reliable data quality

Certified Excellence

10x Genomics CSP using Chromium X with GEM-X for high reproducibility, sensitivity, and efficiency.

Cost-Effective Solutions

State-of-the-art high-throughput platforms and expert support delivering premium data with competitive pricing

Applications of Single Cell Gene Expression Sequencing in Research and Medicine

scRNA-seq is a powerful tool supporting a wide range of research areas, including:

Cancer Research

Uncovering cellular heterogeneity within the tumor microenvironment to reveal cancer mechanisms and potential therapeutic targets.

Immunology

Mapping immune cell diversity and revealing the molecular mechanisms behind immune regulation and dysfunction.

Neuroscience

Deciphering development and regulatory networks to identify potential therapeutic targets for neurological diseases.

Developmental Biology

Charting cell lineage and developmental trajectories to elucidate organ formation and tissue differentiation.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

We support an end-to-end suite of services to ensure that our clients gets the best quality data. This includes a pre-project consultation with our application experts in experimental design, subsequent sample preparation, library construction, sequencing, and bioinformatics analysis.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Demo Results

1/2

Cell Clusters

T-distributed stochastic neighbor embedding (t-SNE) and uniform manifold approximation and projection (UMAP) visualization of cell clusters. Compared to t-SNE, the UMAP visualization tends to have more compact visual clusters with more empty spaces between them.

Note:Each point represents a cell, and each color designates a cluster.

1/1

Expression Pattern of Marker Genes

Heatmaps show the expression of indicated marker genes for given cells and features. In this case, we plotted the top 10 markers (or all markers if less than 10) for each cluster.

Note: Darker red represents a higher expression level, and darker blue dictates a lower expression level.

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Expression Level of Marker Genes

Violin plots show the relative expression levels of marker genes among all clusters. (Only six marker genes are shown here).

Note: The X-axis represents the cluster, and the Y-axis represents the expression level.

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Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Single Cell Gene Expression

High-resolution single-cell transcriptome profiling to reveal cellular heterogeneity and gene expression dynamics across complex populations.

Why Choose Novogene for Single Cell Gene Expression Sequencing?

End-to-End Service

Seamless scRNA-seq workflow from sample processing to bioinformatics with publication-ready outputs

Proven Expertise

High-quality results with industry-leading turnaround and extensive experience across challenging sample types

Enhanced Sample Processing

Flexible processing including nuclei extraction and specialized frozen tissue workflows for reliable data quality

Certified Excellence

10x Genomics CSP using Chromium X with GEM-X for high reproducibility, sensitivity, and efficiency.

Cost-Effective Solutions

State-of-the-art high-throughput platforms and expert support delivering premium data with competitive pricing

Applications of Single Cell Gene Expression Sequencing in Research and Medicine

scRNA-seq is a powerful tool supporting a wide range of research areas, including:

Cancer Research

Uncovering cellular heterogeneity within the tumor microenvironment to reveal cancer mechanisms and potential therapeutic targets.

Immunology

Mapping immune cell diversity and revealing the molecular mechanisms behind immune regulation and dysfunction.

Neuroscience

Deciphering development and regulatory networks to identify potential therapeutic targets for neurological diseases.

Developmental Biology

Charting cell lineage and developmental trajectories to elucidate organ formation and tissue differentiation.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

Project Workflow

Demo Results

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Cell Clusters

Note:Each point represents a cell, and each color designates a cluster.

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Expression Pattern of Marker Genes

Heatmaps show the expression of indicated marker genes for given cells and features. In this case, we plotted the top 10 markers (or all markers if less than 10) for each cluster.

Note: Darker red represents a higher expression level, and darker blue dictates a lower expression level.

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Expression Level of Marker Genes

Violin plots show the relative expression levels of marker genes among all clusters. (Only six marker genes are shown here).

Note: The X-axis represents the cluster, and the Y-axis represents the expression level.

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