Full-Length Transcriptome Sequencing

Comprehensive full-length transcript sequencing to resolve transcript structures, isoform diversity, and gene expression complexity.

Full-length transcriptome sequencing captures complete, unfragmented mRNA molecules. By eliminating the need for bioinformatic transcript assembly, this method delivers a more comprehensive and accurate analysis of novel genes and isoforms. Novogene provides a variety of full-length transcriptome solutions for diverse research objectives.

Full-Length Transcriptome Sequencing (PacBio Iso-Seq): Cost-effective, flexible solutions for the characterization of alternative splicing events, fusion genes, and differentially expressed isoforms with high precision.

Nanopore Direct RNA Sequencing: Sequence mRNA directly in its native form — without conversion to cDNA — to study base modifications (e.g., m6A, m5C) and poly(A) tail characteristics.

Why Choose Novogene for Full-Length Transcriptome Sequencing?

Dedicated Localized Support

Regional technical teams for personalized, responsive service.

Comprehensive Custom Solutions

Tailored project design and analysis for complex samples and challenging studies.

Extensive Experience

Backed by data from over 5,000 samples across 300+ species, our full-length transcriptome sequencing delivers proven reliability.

Guaranteed Data Quality

Our data consistently exceeds manufacturer performance benchmarks.

Applications of Full-Length Transcriptome Sequencing in Research and Medicine

Unlock unbiased and complete RNA profiles to drive discovery in biomedical, agricultural, and life sciences research.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize novel genes, isoforms, and gene fusions. Comprehensively profile alternative splicing to reveal potential biomarkers.

Decipher Disease Mechanisms and Drug Resistance

Investigate the molecular basis of diseases by correlating full-length transcripts with pathological states. Uncover transcriptome-level alterations driving drug resistance in cancer and infectious diseases.

Enhance Genome Annotation

Enable high-quality genome annotation to support evolutionary and functional studies.

Profile RNA Modifications and Poly(A) Dynamics (Direct RNA Sequencing)

Locate key RNA modifications (e.g., m6A) and measure poly(A) tail length to study post-transcriptional regulation.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Demo Results

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Classification of Full-Length Transcripts

The pie chart illustrates the categorization of full-length transcripts.

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Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data

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lncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

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Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

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Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

More Services

mRNA Sequencing

Total RNA Sequencing

Whole Transcriptome Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Full-Length Transcriptome Sequencing

Comprehensive full-length transcript sequencing to resolve transcript structures, isoform diversity, and gene expression complexity.

Nanopore Direct RNA Sequencing: Sequence mRNA directly in its native form — without conversion to cDNA — to study base modifications (e.g., m6A, m5C) and poly(A) tail characteristics.

Why Choose Novogene for Full-Length Transcriptome Sequencing?

Dedicated Localized Support

Regional technical teams for personalized, responsive service.

Comprehensive Custom Solutions

Tailored project design and analysis for complex samples and challenging studies.

Extensive Experience

Backed by data from over 5,000 samples across 300+ species, our full-length transcriptome sequencing delivers proven reliability.

Guaranteed Data Quality

Our data consistently exceeds manufacturer performance benchmarks.

Applications of Full-Length Transcriptome Sequencing in Research and Medicine

Unlock unbiased and complete RNA profiles to drive discovery in biomedical, agricultural, and life sciences research.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize novel genes, isoforms, and gene fusions. Comprehensively profile alternative splicing to reveal potential biomarkers.

Decipher Disease Mechanisms and Drug Resistance

Enhance Genome Annotation

Enable high-quality genome annotation to support evolutionary and functional studies.

Profile RNA Modifications and Poly(A) Dynamics (Direct RNA Sequencing)

Locate key RNA modifications (e.g., m6A) and measure poly(A) tail length to study post-transcriptional regulation.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Service	Amount (Qubit)	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
TotaI RNA	Full-Length Transcriptome Sequencing (PacBio)	≥ 1.2 μg	≥ 40 ng/μL	≥ 6.5 with flat baseline	OD260/280=1.8-2.2; OD260/230=1.3-2.5; *NC/QC ≤ 2.0; No genomic contamination
TotaI RNA	Nanopore Direct RNA Sequencing	≥ 5.5 μg	≧ 375 ng/μL	≥ 7 (animals, plants, fungi); ≥ 8 (Human)	OD260/280=1.8-2.2; OD260/230=1.6-2.5; *NC/QC ≤ 2; No genomic contamination

Note:
*NC/QC: NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform	Full-Length Transcriptome Sequencing (PacBio)	Direct RNA (Nanopore)
Read Length	N50 read length (average) ≥ 1.5 kb	N50 read length (average) ≥ 1 kb
Recommended Data Amount	• 5 million HiFi reads for highly expressed genes/isoforms detection • 10 million HiFi reads for moderate/rare genes/isoforms detection	10M~20M reads/sample (1 PromethION cell/ sample)
Data Analysis Capability	• Data QC • Function Annotation With reference genome • Structure analysis • Alternative splicing • Alternative polyadenylation • Novel gene/ Novel transcript prediction and annotation • Transcription factor analysis • LncRNA prediction • Fusion transcript analysis Without reference genome • Structure analysis • Prediction of coding sequences • Transcription factor analysis • SSR analysis • LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads. • Gene/ Transcript expression level analysis • Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.)	• Full-length transcripts identification and classification • Transcript structure analysis -Alternative splicing -Novel gene/ Novel transcript prediction and annotation -Transcription factor analysis -LncRNA prediction • Quantification analysis • Differential gene expression analysis • GO/KEGG enrichment analysis of differentially expressed genes • RNA modification analysis • Poly(A) analysis *This service is limited to species with a reference genome.

Project Workflow

Our Full-Length Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Demo Results

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Classification of Full-Length Transcripts

The pie chart illustrates the categorization of full-length transcripts.

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Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data

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lncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

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Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

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Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

More Services

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