Plant and Animal Whole Genome Sequencing

Comprehensive whole-genome sequencing of plant and animal species to characterize genetic variation, genome structure, and biological diversity.

Plant and Animal Whole Genome Sequencing (PAWGS) is a powerful technique that enables researchers to sequence the entire genome of an organism. It determines the complete genetic sequence with single-nucleotide resolution. By focusing on resequencing, PAWGS identifies comprehensive genetic variations, including single nucleotide variants (SNVs), insertions and deletions (InDels), copy number variants (CNVs), and structural variants (SVs). These insights facilitate studies in evolution, population genetics, and disease genomics. It is widely applied in fields such as population genetics research, genome-wide association studies (GWAS), animal breeding programs, and agricultural research.

Novogene has extensive experience in Plant and Animal Whole Genome Sequencing across a wide variety of species. We provide PAWGS resequencing services with the flexibility to choose either long-read or short-read sequencing strategies based on your research objectives. Our comprehensive service delivers high-quality data, expert bioinformatics analysis for interpretation, and “publication-ready” figures.

Why Choose Our Plant & Animal Whole Genome Sequencing? What we offer?

Superior Sequencing Data Quality

Advanced sequencing platforms and rigorous QC processes ensure reliable, accurate, and high‑quality results.

Comprehensive Genomic Analysis

Detects SNPs, InDels, CNVs, and SVs to support research in evolution and population genetics.

Excellent Heterozygous Variant Detection

High‑depth, uniform genome coverage enables precise identification of heterozygous variants in complex conditions.

Powerful Bioinformatics & Expert Interpretation

Specialized bioinformatics teams provide customized analyses for GWAS, BSA and population genetics analysis.

Fast Turnaround

High‑quality sequencing data delivered in as little as two weeks to keep your project moving forward efficiently.

Applications of Plant and Animal Whole Genome Sequencing in Research

Unlock the full potential of your research with Novogene's comprehensive whole genome sequencing services. We deliver high-quality and in-depth variation analyses to drive advancements in breeding, conservation, and evolutionary biology.

Comprehensively Identify Functional Variations

Leverage both short- and long-read sequencing to precisely detect the full spectrum of causative SNPs, Indels, and SVs underlying key traits.

Elucidate Evolutionary History and Speciation

Reconstruct species' origins and evolutionary dynamics by uncovering genomic events like gene flow, hybridization, and chromosomal rearrangements.

Characterize Population Structure and Diversity

Profile genetic variation across populations to assess diversity, infer population history, and guide conservation strategies.

Empower Genomic Selection and Improvement

Build foundational genomic resources for accelerated breeding, genomic selection, and the discovery of novel genes for genetic engineering.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Novogene’s Plant & Animal WGS workflow begins with sample quality control to verify DNA purity, integrity, and fragment size. High molecular weight DNA is then used for library preparation, generating either short read or long read libraries depending on project needs. Sequencing is performed using Illumina PE150 or long read platforms to achieve the required depth and coverage for accurate whole genome variant detection. The resulting data undergoes rigorous data quality control, followed by bioinformatics analysis that includes variant calling, annotation, and summary reporting. Final deliverables consist of publication ready FASTQ, BAM/CRAM, VCF files, variant tables, and graphical results to support downstream interpretation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Demo Results

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Structural Variant (SV) Location Overview

This chart provides an overview of the genomic regions where structural variants (SVs) are located.

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SNP Type Distribution

This figure shows the proportion of different types of SNPs found in various genomic regions.

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Comprehensive Genome Mutation Map

This diagram provides a visual map of the genome, precisely locating SNP/InDels and the full spectrum of SV types (INS, DEL, INV, DUP, BND).

More Services

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mRNA Sequencing

Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

Single Cell Gene Expression

Whole Genome Bisulfite Sequencing (WGBS)

Shotgun Metagenomics Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Plant and Animal Whole Genome Sequencing

Comprehensive whole-genome sequencing of plant and animal species to characterize genetic variation, genome structure, and biological diversity.

Why Choose Our Plant & Animal Whole Genome Sequencing? What we offer?

Superior Sequencing Data Quality

Advanced sequencing platforms and rigorous QC processes ensure reliable, accurate, and high‑quality results.

Comprehensive Genomic Analysis

Detects SNPs, InDels, CNVs, and SVs to support research in evolution and population genetics.

Excellent Heterozygous Variant Detection

High‑depth, uniform genome coverage enables precise identification of heterozygous variants in complex conditions.

Powerful Bioinformatics & Expert Interpretation

Specialized bioinformatics teams provide customized analyses for GWAS, BSA and population genetics analysis.

Fast Turnaround

High‑quality sequencing data delivered in as little as two weeks to keep your project moving forward efficiently.

Applications of Plant and Animal Whole Genome Sequencing in Research

Comprehensively Identify Functional Variations

Leverage both short- and long-read sequencing to precisely detect the full spectrum of causative SNPs, Indels, and SVs underlying key traits.

Elucidate Evolutionary History and Speciation

Reconstruct species' origins and evolutionary dynamics by uncovering genomic events like gene flow, hybridization, and chromosomal rearrangements.

Characterize Population Structure and Diversity

Profile genetic variation across populations to assess diversity, infer population history, and guide conservation strategies.

Empower Genomic Selection and Improvement

Build foundational genomic resources for accelerated breeding, genomic selection, and the discovery of novel genes for genetic engineering.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Platform Type	Sample Type	Amount (Qubit®)	Purity
Illumina NovaSeq System	Genomic DNA	≥ 100 ng	A260/280=1.8-2.0;no degradation, no contamination
Illumina NovaSeq System	Genomic DNA (PCR free library)	≥ 1.2 μg	A260/280=1.8-2.0;no degradation, no contamination
PacBio Revio System	HMW Genomic DNA (HiFi SMRTbell® library)	≥ 3.5 μg	A260/280=1.75-2.0; A260/230=1.5-2.6; *NC/QC=1.00-2.20 Fragments should be ≥ 30 kb
Nanopore PromethION	HMW Genomic DNA	≥ 8.5 μg	A260/280=1.75-2.2; A260/230=1.4-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb

*NC/QC = NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis •Data quality control •Sequencing error rate •Filtering reads containing adapter or with low quality •Alignment with reference genome •Statistics of mapping, sequencing depth and coverage •SNP calling, annotation and statistics Advanced Analysis •SV calling, annotation and statistics •CNV calling, annotation and statistics	Standard Analysis •Data quality control •Sequencing alignment •Structural Variant (SV) detection

Project Workflow

Demo Results

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Structural Variant (SV) Location Overview

This chart provides an overview of the genomic regions where structural variants (SVs) are located.

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SNP Type Distribution

This figure shows the proportion of different types of SNPs found in various genomic regions.

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Comprehensive Genome Mutation Map

This diagram provides a visual map of the genome, precisely locating SNP/InDels and the full spectrum of SV types (INS, DEL, INV, DUP, BND).

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