Single Cell Immune Profiling Sequencing

5′ GEX captures transcriptional heterogeneity among intact immune and non-immune cells, facilitating the identification of cell types, activation states, and functional programs. This 10x scRNA-seq–based single-cell RNA-seq approach generates high-resolution single cell gene expression data across complex immune populations. When combined with T cell receptor (TCR) and B cell receptor (BCR) sequencing, this platform enables simultaneous analysis of gene expression and immune clonality, providing insights into clonal expansion and immune cell phenotypes.

At Novogene, our 5′ GEX and immune profiling solutions are powered by the latest 10x Genomics Chromium iX platform and GEM-X chemistry based Universal 5’ Gene Expression assay, delivering high sensitivity, reproducibility, and reliable performance. Our streamlined, end-to-end workflow includes sample processing and quality control, single-cell library preparation, high-throughput sequencing on the NovaSeq X Plus, and comprehensive bioinformatics analysis. We also provide clear, publication-ready visualizations to support intuitive data interpretation and accelerate biological discovery.

Why Choose Novogene for Single Cell Immune Profiling Sequencing?

End-to-End Service

Seamless workflow from sample processing to sequencing and bioinformatics with publication-ready results

High-Quality Data

Guaranteed, industry-standard data with robust QC for consistent and reliable sequencing performance

Comprehensive Bioinformatics Support

Cell Ranger pipelines and customized analysis to uncover immune cell heterogeneity and function

Advanced Technology

Chromium iX with GEM-X enabling state-of-the-art, in-depth immune landscape characterization

Cost-Effective Solutions

High-throughput platforms and expert support delivering premium data with competitive pricing

Applications of Single Cell Immune Profiling in Research and Medicine

Single Cell Immune Profiling has been assisting researchers to analyze immune diversity in the following research areas, including:

Tumor microenvironment

Reveal tumor immune microenvironment characteristics and immunotherapy response mechanisms

Infectious diseases

Analyze pathogen-specific clones to elucidate infection mechanisms and provide evidance for antiviral therapy

Autoimmune diseases

Track clonal evolution of immune repertoires to identify abnormally expanded T-cell or B-cell subpopulations, aiding disease subtyping and target discovery

Candidate vaccines screening

Rapidly screen specific TCR or BCR to accelerate vaccine candidate validation

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Demo Results

1/1

CDR3 Length Profile

Review CDR3 length patterns to support repertoire characterisation and sample comparison.

1/1

Clonotype Count

Compare the number of unique clonotypes across samples or groups to explore immune repertoire diversity.

1/1

V Gene Usage

Visualise V gene usage patterns across samples to identify receptor usage differences within the T cell repertoire.

1/1

Clonal Proportion

Compare the proportion of expanded and low-frequency clonotypes to assess clonal structure across samples.

1/1

V-J Gene Pairing

Visualise V-J gene pairing relationships to support interpretation of receptor recombination and repertoire diversity.

More Services

Single Cell Gene Expression

Visium HD Spatial Gene Expression

Single Cell Long Read Transcriptome

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Single Cell Immune Profiling Sequencing

Why Choose Novogene for Single Cell Immune Profiling Sequencing?

End-to-End Service

Seamless workflow from sample processing to sequencing and bioinformatics with publication-ready results

High-Quality Data

Guaranteed, industry-standard data with robust QC for consistent and reliable sequencing performance

Comprehensive Bioinformatics Support

Cell Ranger pipelines and customized analysis to uncover immune cell heterogeneity and function

Advanced Technology

Chromium iX with GEM-X enabling state-of-the-art, in-depth immune landscape characterization

Cost-Effective Solutions

High-throughput platforms and expert support delivering premium data with competitive pricing

Applications of Single Cell Immune Profiling in Research and Medicine

Single Cell Immune Profiling has been assisting researchers to analyze immune diversity in the following research areas, including:

Tumor microenvironment

Reveal tumor immune microenvironment characteristics and immunotherapy response mechanisms

Infectious diseases

Analyze pathogen-specific clones to elucidate infection mechanisms and provide evidance for antiviral therapy

Autoimmune diseases

Track clonal evolution of immune repertoires to identify abnormally expanded T-cell or B-cell subpopulations, aiding disease subtyping and target discovery

Candidate vaccines screening

Rapidly screen specific TCR or BCR to accelerate vaccine candidate validation

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

Demo Results

1/1

CDR3 Length Profile

Review CDR3 length patterns to support repertoire characterisation and sample comparison.

1/1

Clonotype Count

Compare the number of unique clonotypes across samples or groups to explore immune repertoire diversity.

1/1

V Gene Usage

Visualise V gene usage patterns across samples to identify receptor usage differences within the T cell repertoire.

1/1

Clonal Proportion

Compare the proportion of expanded and low-frequency clonotypes to assess clonal structure across samples.

1/1

V-J Gene Pairing

Visualise V-J gene pairing relationships to support interpretation of receptor recombination and repertoire diversity.

More Services

Single Cell Gene Expression

Visium HD Spatial Gene Expression

Single Cell Long Read Transcriptome

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.