Sequencing Only on Illumina Sequencer

High-quality short-read sequencing of customer-prepared libraries on the Illumina platform to support diverse genomic and transcriptomic applications.

Novogene provides cost-effective sequencing-only services for customer-prepared, ready-to-sequence libraries on the NovaSeq platform series, including NovaSeq X Plus and NovaSeq 6000.

With a flexible range of sequencing solutions, we offer multiple read length options, scalable throughput configurations, and efficient turnaround times to support the diverse needs of research projects.

Supported by an experienced technical and operations team, Novogene combines solid scientific expertise, strict quality control standards, extensive project experience, and streamlined workflows to deliver reliable, efficient sequencing services - providing consistent data quality and dependable support throughout the entire project lifecycle.

Why Choose Illumina Seq-Only Services with Novogene?

Cost Efficiency

Take advantage of industry-leading sequencing costs on a per-read or per-Gb basis, with consistently high Q30 performance.

Trusted Expertise

Rely on Novogene’s experienced team to support you at every stage of sample processing, ensuring consistent, reliable, and high-quality results.

Rapid Turnaround Time

Benefit from our fastest turnaround times at no additional cost, with accelerated processing from quality control to data delivery, helping you meet project deadlines efficiently.

Flexible Project Options

Maintain full control over your sequencing projects with flexible options, including multiple lane or data volume purchases, broad compatibility with diverse library types, and customizable sequencing parameters.

Dedicated Localized Support

Supported by regional technical teams and local laboratories, Novogene delivers personalized and responsive services.

Automated Workflow

Streamline project management using Novogene’s Customer Service System (CSS).

Why Choose Illumina Seq-Only Services with Novogene?

Cost Efficiency

Take advantage of industry-leading sequencing costs on a per-read or per-Gb basis, with consistently high Q30 performance.

Trusted Expertise

Rely on Novogene’s experienced team to support you at every stage of sample processing, ensuring consistent, reliable, and high-quality results.

Rapid Turnaround Time

Benefit from our fastest turnaround times at no additional cost, with accelerated processing from quality control to data delivery, helping you meet project deadlines efficiently.

Flexible Project Options

Dedicated Localized Support

Supported by regional technical teams and local laboratories, Novogene delivers personalized and responsive services.

Automated Workflow

Streamline project management using Novogene’s Customer Service System (CSS).

Applications of Pre-made Library Sequencing

Novogene’s pre-made library sequencing services offer a flexible and cost-effective solution for research teams with established library preparation workflows.

Preserve Proven Workflows

Seamlessly continue validated library preparation protocols while achieving consistent, high-quality sequencing results.

Scale Large and High-Throughput Projects

Support population studies, clinical research, and large cohort analyses with scalable sequencing capacity and optimized costs.

Enable Custom Bioinformatics Pipelines

Receive high-quality raw data (FASTQ or BCL) for direct integration into in-house bioinformatics workflows.

Support Diverse Omics Applications

Applicable to genomics, transcriptomics, targeted sequencing, and other NGS-based research with flexible sequencing configurations.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Project Deliverables

1/1

High-Quality Raw Sequencing Data

Receive high-quality raw sequencing data in FASTQ format. For lane sequencing or flowcell sequencing orders, BCL-format data are also available. All deliverables include a comprehensive quality control (QC) report to ensure data reliability.

1/1

Flexible Bioinformatics Solutions

Optional bioinformatics analysis services are available upon request to support downstream interpretation and discovery, tailored to your research needs.

More Services

Human Whole Genome Sequencing

mRNA Sequencing

Whole Genome Bisulfite Sequencing (WGBS)

Single Cell Gene Expression

Ready to Start Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Sequencing Only on Illumina Sequencer

High-quality short-read sequencing of customer-prepared libraries on the Illumina platform to support diverse genomic and transcriptomic applications.

Novogene provides cost-effective sequencing-only services for customer-prepared, ready-to-sequence libraries on the NovaSeq platform series, including NovaSeq X Plus and NovaSeq 6000.

Why Choose Illumina Seq-Only Services with Novogene?

Cost Efficiency

Take advantage of industry-leading sequencing costs on a per-read or per-Gb basis, with consistently high Q30 performance.

Trusted Expertise

Rely on Novogene’s experienced team to support you at every stage of sample processing, ensuring consistent, reliable, and high-quality results.

Rapid Turnaround Time

Benefit from our fastest turnaround times at no additional cost, with accelerated processing from quality control to data delivery, helping you meet project deadlines efficiently.

Flexible Project Options

Dedicated Localized Support

Supported by regional technical teams and local laboratories, Novogene delivers personalized and responsive services.

Automated Workflow

Streamline project management using Novogene’s Customer Service System (CSS).

Why Choose Illumina Seq-Only Services with Novogene?

Cost Efficiency

Take advantage of industry-leading sequencing costs on a per-read or per-Gb basis, with consistently high Q30 performance.

Trusted Expertise

Rely on Novogene’s experienced team to support you at every stage of sample processing, ensuring consistent, reliable, and high-quality results.

Rapid Turnaround Time

Benefit from our fastest turnaround times at no additional cost, with accelerated processing from quality control to data delivery, helping you meet project deadlines efficiently.

Flexible Project Options

Dedicated Localized Support

Supported by regional technical teams and local laboratories, Novogene delivers personalized and responsive services.

Automated Workflow

Streamline project management using Novogene’s Customer Service System (CSS).

Applications of Pre-made Library Sequencing

Novogene’s pre-made library sequencing services offer a flexible and cost-effective solution for research teams with established library preparation workflows.

Preserve Proven Workflows

Seamlessly continue validated library preparation protocols while achieving consistent, high-quality sequencing results.

Scale Large and High-Throughput Projects

Support population studies, clinical research, and large cohort analyses with scalable sequencing capacity and optimized costs.

Enable Custom Bioinformatics Pipelines

Receive high-quality raw data (FASTQ or BCL) for direct integration into in-house bioinformatics workflows.

Support Diverse Omics Applications

Applicable to genomics, transcriptomics, targeted sequencing, and other NGS-based research with flexible sequencing configurations.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Platform	Sequencing Platform	Data Amount	Volume	Concentration*
NovaSeq X Plus PE150/PE50/SE50	NovaSeq X Plus Partial Lane Seq	X < 30 G	≥ 15 µL	≥ 2 ng/L, quantified by Qubit® 2.0 (Life Technologies) or ≥ 2 nmol/L quantified by Q-PCR
		30 G ≤ X < 100 G	≥ 30 µL
		100 G ≤ X < 375 G	≥ 70 µL
	NovaSeq X Plus 10B	Lane sequencing (375Gb data per lane)	≥70 μL/lane (add 70 µL for each additional lane)
	NovaSeq X Plus 25B	Lane sequencing (1,000 Gb data per lane)	≥ 130 μL/lane (add 130 µL for each additional lane)
NovaSeq6000 PE250	NovaSeq 6000 Partial Lane Seq	X < 30 M reads	≥ 15 µL
		30 M ≤ X < 100 M reads	≥ 25 µL
		100 M ≤ X < 400 M reads	≥ 50 µL
	NovaSeq 6000 Lane Seq	Lane sequencing (400 M reads per lane)	≥ 70 µL/lane (add 70 µL for each additional lane)

*Notes:High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Sequencing and Analysis

Platform	Read Length	Data Output
NovaSeq X Plus	PE150 (10B)	375Gb/lane 3 Tb/flow cell
	PE150 (25B)	1 Tb/lane 8 Tb/flow cell
	PE50	1.5B reads/flowcell
	SE50	1.5B reads/flowcell
NovaSeq 6000	PE250	400 M pair reads/lane 800 M pair reads/flowcell

Project Workflow

Project Deliverables

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