DM-seq (Directed DNA Methylation Sequencing) is an advanced DNA methylation solution that addresses core limitations of traditional methods like whole‑genome bisulfite sequencing. By directly converting methylated cytosine (C) information into thymine (T) signals using Watchmaker TAPS+ and Illumina 5-base technology, DM-seq enables simultaneous detection of DNA methylation profiles and genomic variations (SNP/Indel/SV/CNV) from a single library. The enzymatic conversion also reduces input requirements, making it ideal for challenging samples such as cfDNA and FFPE.
This novel approach represents a cost‑effective strategy for multi‑omics analysis, enabling researchers to extract both genetic and epigenetic information from a single DNA library and further supporting a wide range of applications, including disease mechanism exploration, tumor precision medicine, rare disease etiology, agricultural trait improvement, stress resistance breeding, and basic developmental biology research.
