mRNA Sequencing

Genome-wide profiling of messenger RNA expression to reveal coding transcript dynamics and gene regulatory changes across biological conditions.

mRNA sequencing (RNA-Seq) is a powerful tool for capturing high-resolution transcriptional dynamics. It provides accurate data essential for advancing research—from identifying alternative splicing events, novel genes, and RNA sequence variants to profiling gene expression patterns—in areas such as functional genomics, disease mechanism studies, and biomarker discovery.

Novogene provides fully integrated, end-to-end RNA-Seq solutions. Our workflow spans the entire process—from RNA extraction and library preparation to sequencing and comprehensive bioinformatic analysis. Supported by one of the industry’s largest sequencing capacities and rigorous stage-by-stage quality control, we leverage cutting-edge Illumina NovaSeq platforms to deliver reliable, high-quality data on accelerated timelines. Our NovoMagic cloud platform further enables you to transform this data into actionable biological insight, allowing you to easily visualize, customize, and reanalyze results within a single platform.

Why Choose Novogene for Your mRNA-seq Needs?

Extensive Experience

Processing over 1 million RNA-seq samples annually with reliable, refined workflows.

Cloud Bioinformatics Platform-NovoMagic

Conduct rapid, self-directed analysis and visualization, anytime, anywhere.

Dedicated Localized Support

Regional technical teams and labs for personalized, responsive service.

Comprehensive Custom Solutions

Tailored project design for complex samples and challenging studies.

Rapid and Reliable Turnaround

Data delivery starting at two weeks from sample receipt.

Guaranteed Data Quality

Our data consistently exceeds manufacturer performance benchmarks.

Applications of mRNA-Seq in Research and Medicine

Discover the power of mRNA-seq with Novogene’s services, designed to assist in a variety of research goals

Explore Diversity

Understand transcript profiles across different tissues, conditions, and treatments.

Uncover Novel Insights

Identify new transcripts, alternative splicing, and variations for a comprehensive view.

Navigate Development

Study developmental mechanisms and drug resistance through time-course gene expression.

Find Biomarkers

Discover potential biomarkers using novel transcripts, SNP/InDel identification, and fusion gene discovery.

Comprehensive Combining

Combine transcriptome data with omics approaches for a holistic perspective.

Clinical Insight

Investigate pathogenic mechanisms and clinical subtypes for precise clinical diagnosis.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Resources

Demo Results

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Volcano Plot of Differentially Expressed Genes (DEGs)

Volcano plot of significantly up- and down-regulated genes.

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Heatmap of Differentially Expressed Genes (DEGs)

Clustered expression profiles of DEGs across different sample groups.

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KEGG Pathway Enrichment Analysis

Enriched biological pathways among DEGs, based on the KEGG database.

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Hierarchical Clustering of DEGs

Genes within the same cluster show coordinated expression changes across treatment conditions.

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Alternative Splicing Visualization

Differential alternative splicing events identified by rMATS software.

Frequently Asked Questions

How much data do l need for RNA-seq?

The ideal data amount, depends on the specific research goal. Below are general guidelines for two common scenarios:

•For standard differential expression analysis: 20 million reads per sample (with ≥3 biological replicates) are often sufficient.

•For detecting lowly expressed genes (in-depth profiling): 30–60 million reads per sample are recommended, starting with at least 30 million and including adequate replicates.

How many replicates are required?

When should I choose strand-specific RNA-seq?

What precautions should l take when sending samples for RNA-seq?

What is the difference between bulk RNA-seq and single-cell RNA-seq?

More Services

Single Cell Gene Expression

Visium HD Spatial Gene Expression

Full-Length Transcriptome Sequencing

Total RNA Sequencing

Small RNA Sequencing

Circular RNA Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

mRNA Sequencing

Genome-wide profiling of messenger RNA expression to reveal coding transcript dynamics and gene regulatory changes across biological conditions.

Why Choose Novogene for Your mRNA-seq Needs?

Extensive Experience

Processing over 1 million RNA-seq samples annually with reliable, refined workflows.

Cloud Bioinformatics Platform-NovoMagic

Conduct rapid, self-directed analysis and visualization, anytime, anywhere.

Dedicated Localized Support

Regional technical teams and labs for personalized, responsive service.

Comprehensive Custom Solutions

Tailored project design for complex samples and challenging studies.

Rapid and Reliable Turnaround

Data delivery starting at two weeks from sample receipt.

Guaranteed Data Quality

Our data consistently exceeds manufacturer performance benchmarks.

Applications of mRNA-Seq in Research and Medicine

Discover the power of mRNA-seq with Novogene’s services, designed to assist in a variety of research goals

Explore Diversity

Understand transcript profiles across different tissues, conditions, and treatments.

Uncover Novel Insights

Identify new transcripts, alternative splicing, and variations for a comprehensive view.

Navigate Development

Study developmental mechanisms and drug resistance through time-course gene expression.

Find Biomarkers

Discover potential biomarkers using novel transcripts, SNP/InDel identification, and fusion gene discovery.

Comprehensive Combining

Combine transcriptome data with omics approaches for a holistic perspective.

Clinical Insight

Investigate pathogenic mechanisms and clinical subtypes for precise clinical diagnosis.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	RIN (Agilent 2100)	Purity (NanoDrop™)
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 200 ng	≥ 20 μL	≥ 10 ng/μL	≥ 4.0, with flat baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination
Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Animal/Plant/Fungus)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline
Strand-specific Eukaryotic mRNA (PolyA enrichment)	Total RNA (Blood)	≥ 400 ng	≥ 20 μL	≥ 20 ng/μL	≥ 5.0, with flat baseline

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150 bp
Recommended Data Amount	• ≥ 20 million read pairs per sample for species with reference genome • ≥ 50 million read pairs per sample for species without reference genome (de novo transcriptome assembly projects)
Data Analysis Capability	mRNA-seq analysis with reference genome •Data quality control •Gene expression quantification •Differential expression profiling •Functional enrichment analysis •Novel gene prediction •SNP & InDel analysis •Alternative splicing (AS) analysis •Fusion gene prediction •Protein-Protein Interaction (PPI) analysis •Oncogene functional annotation
Data Analysis Capability	mRNA-seq analysis without reference genome •Data quality control •De novo transcriptome assembly •Gene functional annotation •CDS prediction •SSR analysis •Gene expression quantification •Differential expression profiling •Functional enrichment analysis

Project Workflow

Our mRNA-Seq workflow ensures high-quality results through a rigorous, step-by-step process:

Resources

Demo Results

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Volcano Plot of Differentially Expressed Genes (DEGs)

Volcano plot of significantly up- and down-regulated genes.

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Heatmap of Differentially Expressed Genes (DEGs)

Clustered expression profiles of DEGs across different sample groups.

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KEGG Pathway Enrichment Analysis

Enriched biological pathways among DEGs, based on the KEGG database.

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Hierarchical Clustering of DEGs

Genes within the same cluster show coordinated expression changes across treatment conditions.

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Alternative Splicing Visualization

Differential alternative splicing events identified by rMATS software.

Frequently Asked Questions

How much data do l need for RNA-seq?

The ideal data amount, depends on the specific research goal. Below are general guidelines for two common scenarios:

•For standard differential expression analysis: 20 million reads per sample (with ≥3 biological replicates) are often sufficient.

•For detecting lowly expressed genes (in-depth profiling): 30–60 million reads per sample are recommended, starting with at least 30 million and including adequate replicates.

How many replicates are required?

When should I choose strand-specific RNA-seq?

What precautions should l take when sending samples for RNA-seq?

What is the difference between bulk RNA-seq and single-cell RNA-seq?

More Services

Single Cell Gene Expression

Visium HD Spatial Gene Expression

Full-Length Transcriptome Sequencing

Total RNA Sequencing

Small RNA Sequencing

Circular RNA Sequencing

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