Novogene AMEA
  • Novogene AMEA
  • Genomics
    • Human Whole Genome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Microbial Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial De novo Sequencing
    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing
    • Whole Exome Sequencing
    Transcriptomics
    • mRNA Sequencing
    • Total RNA Sequencing
    • Full-Length Transcriptome Sequencing
    • Whole Transcriptome Sequencing
    • Small RNA Sequencing
    • Circular RNA Sequencing
    • Metatranscriptome Sequencing
    • Prokaryotic RNA Sequencing
    Single Cell & Spatial Omics
    • Single Cell Gene Expression
    • Single Cell Immune Profiling Sequencing
    • Single Cell Long Read Transcriptome
    • Visium HD Spatial Gene Expression
    • Stereo-Seq Spatial Gene Expression
    • Xenium In Situ Spatial Transcriptome
    Epigenomics
    • Whole Genome Bisulfite Sequencing (WGBS)
    • Directed DNA Methylation Sequencing (DM-Seq) NEW
    • Reduced Representation Bisulfite Sequencing (RRBS)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on PacBio Sequencer
    Proteomics and Metabolomics
    • Olink Proteomics
    • Quantitative Proteomics
    • Untargeted Metabolomics
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    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
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    • Case Studies
    • Webinar
    • Blog
    • Sample Guidelines
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • Our Locations
    • News
    • Careers
  • Contact UsContact Us

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Services
Whole Genome SequencingDe novo SequencingAmplicon SequencingShotgun Metagenomic SequencingDirected DNA Methylation Sequencing (DM-Seq)mRNA SequencingSingle Cell Gene ExpressionVisium HD Spatial Gene ExpressionXenium In Situ Spatial TranscriptomeOlink ProteomicsUntargeted Metabolomics
Support
NovoMagic Bioinformatics Analysis ToolCustomer Service SystemFalcon Intelligent Delivery Platform
Company
About UsOur LocationsOur PlatformsNewsCareersContact Us
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Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
Novogene AMEA
  • Novogene AMEA
  • Genomics
    • Human Whole Genome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Microbial Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial De novo Sequencing
    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing
    • Whole Exome Sequencing
    Transcriptomics
    • mRNA Sequencing
    • Total RNA Sequencing
    • Full-Length Transcriptome Sequencing
    • Whole Transcriptome Sequencing
    • Small RNA Sequencing
    • Circular RNA Sequencing
    • Metatranscriptome Sequencing
    • Prokaryotic RNA Sequencing
    Single Cell & Spatial Omics
    • Single Cell Gene Expression
    • Single Cell Immune Profiling Sequencing
    • Single Cell Long Read Transcriptome
    • Visium HD Spatial Gene Expression
    • Stereo-Seq Spatial Gene Expression
    • Xenium In Situ Spatial Transcriptome
    Epigenomics
    • Whole Genome Bisulfite Sequencing (WGBS)
    • Directed DNA Methylation Sequencing (DM-Seq) NEW
    • Reduced Representation Bisulfite Sequencing (RRBS)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on PacBio Sequencer
    Proteomics and Metabolomics
    • Olink Proteomics
    • Quantitative Proteomics
    • Untargeted Metabolomics
  • PromotionsPromotions
    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Brochures
    • Case Studies
    • Webinar
    • Blog
    • Sample Guidelines
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • Our Locations
    • News
    • Careers
  • Contact UsContact Us

ServicesServices menu

SupportSupport menu

CompanyCompany menu

Services
Whole Genome SequencingDe novo SequencingAmplicon SequencingShotgun Metagenomic SequencingDirected DNA Methylation Sequencing (DM-Seq)mRNA SequencingSingle Cell Gene ExpressionVisium HD Spatial Gene ExpressionXenium In Situ Spatial TranscriptomeOlink ProteomicsUntargeted Metabolomics
Support
NovoMagic Bioinformatics Analysis ToolCustomer Service SystemFalcon Intelligent Delivery Platform
Company
About UsOur LocationsOur PlatformsNewsCareersContact Us
LinkedInLinkedIn hoverYouTubeYouTube hoverXX hover
Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
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Whole Genome Bisulfite Sequencing (WGBS)

Genome-wide, single-base resolution profiling of DNA methylation to reveal epigenetic regulation and methylation patterns across the genome.
Request a Quote
(Whole Genome Bisulfite Sequencing (WGBS))Request a Quote
(Whole Genome Bisulfite Sequencing (WGBS))
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodeling. Perturbations in methylation patterns are associated with tumorigenesis, neurodegenerative diseases and neurological disorders. Bioinformatic analysis of methylomes is widely used in various research areas, including studies on gene regulation, cell differentiation, embryogenesis, aging, occurrence and development of disease, phenotypic diversity and evolution in plants and animals.


Novogene provides whole genome bisulfite sequencing (WGBS) to efficiently identify methylated cytosines on a genome-wide scale with a single nucleotide resolution. The deep reach of the methylome is imperative for understanding gene expression and multiple biological processes that are subject to epigenetic regulation.

Benefits

Profound Experience
Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Profound Experience
Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Low input and high efficiency
Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Low input and high efficiency
Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Comprehensive Analysis
Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Comprehensive Analysis
Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Customized solutions
Customized solutions

Customized association analysis can explore correlations between methylation and gene expression

Customized solutions
Customized solutions

Customized association analysis can explore correlations between methylation and gene expression

Rapid and High Capacity
Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable industry-leading turnaround times.

Rapid and High Capacity
Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable industry-leading turnaround times.

Benefits

Profound Experience
Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Profound Experience
Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Low input and high efficiency
Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Low input and high efficiency
Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Comprehensive Analysis
Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Comprehensive Analysis
Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Customized solutions
Customized solutions

Customized association analysis can explore correlations between methylation and gene expression

Customized solutions
Customized solutions

Customized association analysis can explore correlations between methylation and gene expression

Rapid and High Capacity
Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable industry-leading turnaround times.

Rapid and High Capacity
Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable industry-leading turnaround times.

Applications

Novogene delivers high-quality data and publication-ready analysis results of WGBS services, to faciliate research including:

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Applications

Novogene delivers high-quality data and publication-ready analysis results of WGBS services, to faciliate research including:

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Demo Results

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Distribution of Genome Coverage
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Methylation Level Distribution on Whole Genome
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Heatmap Analysis for Methylation Levels of Gene Functional Region
Image
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Methylation Level Distribution at Functional Genetic Elements
Image
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1/1
Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)
Image
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1/1
Diagram of GO Enrichment
Image
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1/1
Distribution of Genome Coverage
Image
Image
1/1
Methylation Level Distribution on Whole Genome
Image
Image
1/1
Heatmap Analysis for Methylation Levels of Gene Functional Region
Image
Image
1/1
Methylation Level Distribution at Functional Genetic Elements
Image
Image
1/1
Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)
Image
Image
1/1
Diagram of GO Enrichment

Demo Results

Image
Image
1/1
Distribution of Genome Coverage
Image
Image
1/1
Methylation Level Distribution on Whole Genome
Image
Image
1/1
Heatmap Analysis for Methylation Levels of Gene Functional Region
Image
Image
1/1
Methylation Level Distribution at Functional Genetic Elements
Image
Image
1/1
Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)
Image
Image
1/1
Diagram of GO Enrichment
Image
Image
1/1
Distribution of Genome Coverage
Image
Image
1/1
Methylation Level Distribution on Whole Genome
Image
Image
1/1
Heatmap Analysis for Methylation Levels of Gene Functional Region
Image
Image
1/1
Methylation Level Distribution at Functional Genetic Elements
Image
Image
1/1
Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)
Image
Image
1/1
Diagram of GO Enrichment

More Services

Chromatin Immunoprecipitation Sequencing (ChIP-seq)
(Chromatin Immunoprecipitation Sequencing (ChIP-seq))
Chromatin Immunoprecipitation Sequencing (ChIP-seq)
(Chromatin Immunoprecipitation Sequencing (ChIP-seq))
RNA Immunoprecipitation Sequencing (RIP-seq)
(RNA Immunoprecipitation Sequencing (RIP-seq))
RNA Immunoprecipitation Sequencing (RIP-seq)
(RNA Immunoprecipitation Sequencing (RIP-seq))
Reduced Representation Bisulfite Sequencing (RRBS)
(Reduced Representation Bisulfite Sequencing (RRBS))
Reduced Representation Bisulfite Sequencing (RRBS)
(Reduced Representation Bisulfite Sequencing (RRBS))

More Services

Chromatin Immunoprecipitation Sequencing (ChIP-seq)
(Chromatin Immunoprecipitation Sequencing (ChIP-seq))
Chromatin Immunoprecipitation Sequencing (ChIP-seq)
(Chromatin Immunoprecipitation Sequencing (ChIP-seq))
RNA Immunoprecipitation Sequencing (RIP-seq)
(RNA Immunoprecipitation Sequencing (RIP-seq))
RNA Immunoprecipitation Sequencing (RIP-seq)
(RNA Immunoprecipitation Sequencing (RIP-seq))
Reduced Representation Bisulfite Sequencing (RRBS)
(Reduced Representation Bisulfite Sequencing (RRBS))
Reduced Representation Bisulfite Sequencing (RRBS)
(Reduced Representation Bisulfite Sequencing (RRBS))
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Whole Genome Bisulfite Sequencing (WGBS)

Genome-wide, single-base resolution profiling of DNA methylation to reveal epigenetic regulation and methylation patterns across the genome.
Request a Quote
(Whole Genome Bisulfite Sequencing (WGBS))Request a Quote
(Whole Genome Bisulfite Sequencing (WGBS))
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodeling. Perturbations in methylation patterns are associated with tumorigenesis, neurodegenerative diseases and neurological disorders. Bioinformatic analysis of methylomes is widely used in various research areas, including studies on gene regulation, cell differentiation, embryogenesis, aging, occurrence and development of disease, phenotypic diversity and evolution in plants and animals.


Novogene provides whole genome bisulfite sequencing (WGBS) to efficiently identify methylated cytosines on a genome-wide scale with a single nucleotide resolution. The deep reach of the methylome is imperative for understanding gene expression and multiple biological processes that are subject to epigenetic regulation.

Benefits

Profound Experience
Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Profound Experience
Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Low input and high efficiency
Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Low input and high efficiency
Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Comprehensive Analysis
Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Comprehensive Analysis
Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Customized solutions
Customized solutions

Customized association analysis can explore correlations between methylation and gene expression

Customized solutions
Customized solutions

Customized association analysis can explore correlations between methylation and gene expression

Rapid and High Capacity
Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable industry-leading turnaround times.

Rapid and High Capacity
Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable industry-leading turnaround times.

Benefits

Profound Experience
Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Profound Experience
Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Low input and high efficiency
Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Low input and high efficiency
Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Comprehensive Analysis
Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Comprehensive Analysis
Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Customized solutions
Customized solutions

Customized association analysis can explore correlations between methylation and gene expression

Customized solutions
Customized solutions

Customized association analysis can explore correlations between methylation and gene expression

Rapid and High Capacity
Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable industry-leading turnaround times.

Rapid and High Capacity
Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable industry-leading turnaround times.

Applications

Novogene delivers high-quality data and publication-ready analysis results of WGBS services, to faciliate research including:

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Applications

Novogene delivers high-quality data and publication-ready analysis results of WGBS services, to faciliate research including:

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Sample TypeRequired AmountPurity
Genomic DNA≥ 200 ng1.8<A260/280<2.0
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired end 150 bp
Recommended sequencing depth≥ 30× coverage for species with reference genome
Standard Data Analysis· Data Quality Control
· mCs detection, methylation level calculation
· Methylation level and frequency distribution
· Differentially Methylated Site (DMS) detection
· Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
· Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data
· Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Project Workflow

Demo Results

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Distribution of Genome Coverage
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Methylation Level Distribution on Whole Genome
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Heatmap Analysis for Methylation Levels of Gene Functional Region
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Methylation Level Distribution at Functional Genetic Elements
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Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)
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Diagram of GO Enrichment
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Distribution of Genome Coverage
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Methylation Level Distribution on Whole Genome
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Heatmap Analysis for Methylation Levels of Gene Functional Region
Image
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Methylation Level Distribution at Functional Genetic Elements
Image
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Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)
Image
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Diagram of GO Enrichment

Demo Results

Image
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Distribution of Genome Coverage
Image
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1/1
Methylation Level Distribution on Whole Genome
Image
Image
1/1
Heatmap Analysis for Methylation Levels of Gene Functional Region
Image
Image
1/1
Methylation Level Distribution at Functional Genetic Elements
Image
Image
1/1
Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)
Image
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1/1
Diagram of GO Enrichment
Image
Image
1/1
Distribution of Genome Coverage
Image
Image
1/1
Methylation Level Distribution on Whole Genome
Image
Image
1/1
Heatmap Analysis for Methylation Levels of Gene Functional Region
Image
Image
1/1
Methylation Level Distribution at Functional Genetic Elements
Image
Image
1/1
Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)
Image
Image
1/1
Diagram of GO Enrichment

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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
(Chromatin Immunoprecipitation Sequencing (ChIP-seq))
Chromatin Immunoprecipitation Sequencing (ChIP-seq)
(Chromatin Immunoprecipitation Sequencing (ChIP-seq))
RNA Immunoprecipitation Sequencing (RIP-seq)
(RNA Immunoprecipitation Sequencing (RIP-seq))
RNA Immunoprecipitation Sequencing (RIP-seq)
(RNA Immunoprecipitation Sequencing (RIP-seq))
Reduced Representation Bisulfite Sequencing (RRBS)
(Reduced Representation Bisulfite Sequencing (RRBS))
Reduced Representation Bisulfite Sequencing (RRBS)
(Reduced Representation Bisulfite Sequencing (RRBS))
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