Novogene AMEA
  • Novogene AMEA
  • Genomics
    • Human Whole Genome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Microbial Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial De novo Sequencing
    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing
    • Whole Exome Sequencing
    Transcriptomics
    • mRNA Sequencing
    • Total RNA Sequencing
    • Full-Length Transcriptome Sequencing
    • Whole Transcriptome Sequencing
    • Small RNA Sequencing
    • Circular RNA Sequencing
    • Metatranscriptome Sequencing
    • Prokaryotic RNA Sequencing
    Single Cell & Spatial Omics
    • Single Cell Gene Expression
    • Single Cell Immune Profiling Sequencing
    • Single Cell Long Read Transcriptome
    • Visium HD Spatial Gene Expression
    • Stereo-Seq Spatial Gene Expression
    • Xenium In Situ Spatial Transcriptome
    Epigenomics
    • Whole Genome Bisulfite Sequencing (WGBS)
    • Directed DNA Methylation Sequencing (DM-Seq) NEW
    • Reduced Representation Bisulfite Sequencing (RRBS)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on PacBio Sequencer
    Proteomics and Metabolomics
    • Olink Proteomics
    • Quantitative Proteomics
    • Untargeted Metabolomics
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    • Rare and Complex Diseases
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Whole Genome SequencingDe novo SequencingAmplicon SequencingShotgun Metagenomic SequencingDirected DNA Methylation Sequencing (DM-Seq)mRNA SequencingSingle Cell Gene ExpressionVisium HD Spatial Gene ExpressionXenium In Situ Spatial TranscriptomeOlink ProteomicsUntargeted Metabolomics
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Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
Novogene AMEA
  • Novogene AMEA
  • Genomics
    • Human Whole Genome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Microbial Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial De novo Sequencing
    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing
    • Whole Exome Sequencing
    Transcriptomics
    • mRNA Sequencing
    • Total RNA Sequencing
    • Full-Length Transcriptome Sequencing
    • Whole Transcriptome Sequencing
    • Small RNA Sequencing
    • Circular RNA Sequencing
    • Metatranscriptome Sequencing
    • Prokaryotic RNA Sequencing
    Single Cell & Spatial Omics
    • Single Cell Gene Expression
    • Single Cell Immune Profiling Sequencing
    • Single Cell Long Read Transcriptome
    • Visium HD Spatial Gene Expression
    • Stereo-Seq Spatial Gene Expression
    • Xenium In Situ Spatial Transcriptome
    Epigenomics
    • Whole Genome Bisulfite Sequencing (WGBS)
    • Directed DNA Methylation Sequencing (DM-Seq) NEW
    • Reduced Representation Bisulfite Sequencing (RRBS)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on PacBio Sequencer
    Proteomics and Metabolomics
    • Olink Proteomics
    • Quantitative Proteomics
    • Untargeted Metabolomics
  • PromotionsPromotions
    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Brochures
    • Case Studies
    • Webinar
    • Blog
    • Sample Guidelines
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • Our Locations
    • News
    • Careers
  • Contact UsContact Us

ServicesServices menu

SupportSupport menu

CompanyCompany menu

Services
Whole Genome SequencingDe novo SequencingAmplicon SequencingShotgun Metagenomic SequencingDirected DNA Methylation Sequencing (DM-Seq)mRNA SequencingSingle Cell Gene ExpressionVisium HD Spatial Gene ExpressionXenium In Situ Spatial TranscriptomeOlink ProteomicsUntargeted Metabolomics
Support
NovoMagic Bioinformatics Analysis ToolCustomer Service SystemFalcon Intelligent Delivery Platform
Company
About UsOur LocationsOur PlatformsNewsCareersContact Us
LinkedInLinkedIn hoverYouTubeYouTube hoverXX hover
Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
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Whole Transcriptome Sequencing

Comprehensive sequencing of the whole transcriptome to characterize coding and non-coding RNA expression, transcript structure, and regulatory complexity.
Request a Quote
(Whole Transcriptome Sequencing)
Request a Quote
(Whole Transcriptome Sequencing)
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Whole Transcriptome Sequencing is a powerful approach that profiles all types of RNA molecules in a biological sample, including mRNA, non-coding RNAs. It provides a comprehensive view of the transcriptome.

Novogene’s Whole Transcriptome Sequencing offers a holistic analysis of transcriptional activity. It enables the simultaneous identification and quantification of diverse RNA species, facilitates the exploration of complex RNA interactions and regulatory networks, allows in-depth investigation of how different RNA molecules coordinate in biological processes, disease development, and cellular functions, and enhances our understanding of the multi-layered regulation of gene expression.

Benefits of Whole Transcriptome Sequencing

Comprehensive Transcript Detection
Comprehensive Transcript Detection

Achieve simultaneous identification and quantification of all RNA types, including mRNA, lncRNA, sRNA, circRNA, with high sensitivity and precision.

Comprehensive Transcript Detection
Comprehensive Transcript Detection

Achieve simultaneous identification and quantification of all RNA types, including mRNA, lncRNA, sRNA, circRNA, with high sensitivity and precision.

Vast Research Expertise
Vast Research Expertise

Leverage extensive whole transcriptome sequencing project experience to support in-depth investigations into gene expression regulation, biological processes, and disease mechanisms.

Vast Research Expertise
Vast Research Expertise

Leverage extensive whole transcriptome sequencing project experience to support in-depth investigations into gene expression regulation, biological processes, and disease mechanisms.

Integrated Multi-Omics Analysis Solutions
Integrated Multi-Omics Analysis Solutions

Obtain all-round analysis covering transcript identification, expression profiling, differential expression analysis, functional annotation, and regulatory network construction across multiple RNA species.

Integrated Multi-Omics Analysis Solutions
Integrated Multi-Omics Analysis Solutions

Obtain all-round analysis covering transcript identification, expression profiling, differential expression analysis, functional annotation, and regulatory network construction across multiple RNA species.

Publication-Ready Data Curation
Publication-Ready Data Curation

Receive specialized bioinformatics support to generate publication-quality results, enabling seamless submission to high-impact scientific journals.

Publication-Ready Data Curation
Publication-Ready Data Curation

Receive specialized bioinformatics support to generate publication-quality results, enabling seamless submission to high-impact scientific journals.

Benefits of Whole Transcriptome Sequencing

Comprehensive Transcript Detection
Comprehensive Transcript Detection

Achieve simultaneous identification and quantification of all RNA types, including mRNA, lncRNA, sRNA, circRNA, with high sensitivity and precision.

Comprehensive Transcript Detection
Comprehensive Transcript Detection

Achieve simultaneous identification and quantification of all RNA types, including mRNA, lncRNA, sRNA, circRNA, with high sensitivity and precision.

Vast Research Expertise
Vast Research Expertise

Leverage extensive whole transcriptome sequencing project experience to support in-depth investigations into gene expression regulation, biological processes, and disease mechanisms.

Vast Research Expertise
Vast Research Expertise

Leverage extensive whole transcriptome sequencing project experience to support in-depth investigations into gene expression regulation, biological processes, and disease mechanisms.

Integrated Multi-Omics Analysis Solutions
Integrated Multi-Omics Analysis Solutions

Obtain all-round analysis covering transcript identification, expression profiling, differential expression analysis, functional annotation, and regulatory network construction across multiple RNA species.

Integrated Multi-Omics Analysis Solutions
Integrated Multi-Omics Analysis Solutions

Obtain all-round analysis covering transcript identification, expression profiling, differential expression analysis, functional annotation, and regulatory network construction across multiple RNA species.

Publication-Ready Data Curation
Publication-Ready Data Curation

Receive specialized bioinformatics support to generate publication-quality results, enabling seamless submission to high-impact scientific journals.

Publication-Ready Data Curation
Publication-Ready Data Curation

Receive specialized bioinformatics support to generate publication-quality results, enabling seamless submission to high-impact scientific journals.

Applications of Whole Transcriptome Sequencing

Whole transcriptome sequencing has emerged as a powerful tool to explore the comprehensive landscape of all RNA molecules in a biological system, with the following prominent applications:

Pan-RNA Identification and Profiling

It enables the discovery of novel transcripts across all RNA types, accurate quantification of their expression levels, and characterization of their functional features.

Pan-RNA Identification and Profiling

It enables the discovery of novel transcripts across all RNA types, accurate quantification of their expression levels, and characterization of their functional features.

Disease Mechanism and Biomarker Discovery

By profiling multi-type RNA expression patterns in physiological and pathological contexts, it helps identify diagnostic, prognostic, and predictive biomarkers, and elucidate molecular mechanisms underlying various diseases.

Disease Mechanism and Biomarker Discovery

By profiling multi-type RNA expression patterns in physiological and pathological contexts, it helps identify diagnostic, prognostic, and predictive biomarkers, and elucidate molecular mechanisms underlying various diseases.

Transcriptional and Post-Transcriptional Regulation Elucidation

It facilitates the investigation of gene regulation networks, including transcriptional control, mRNA splicing, non-coding RNA-mediated regulation, and their crosstalk, thereby shedding light on complex regulatory landscapes.

Transcriptional and Post-Transcriptional Regulation Elucidation

It facilitates the investigation of gene regulation networks, including transcriptional control, mRNA splicing, non-coding RNA-mediated regulation, and their crosstalk, thereby shedding light on complex regulatory landscapes.

Therapeutic Target and Functional Study Tool

It serves as a foundation for exploring diverse RNA molecules as potential therapeutic targets. Also, it aids in functional studies to elucidate how different RNA species participate in cellular processes, development, and disease progression.

Therapeutic Target and Functional Study Tool

It serves as a foundation for exploring diverse RNA molecules as potential therapeutic targets. Also, it aids in functional studies to elucidate how different RNA species participate in cellular processes, development, and disease progression.

Applications of Whole Transcriptome Sequencing

Whole transcriptome sequencing has emerged as a powerful tool to explore the comprehensive landscape of all RNA molecules in a biological system, with the following prominent applications:

Pan-RNA Identification and Profiling

It enables the discovery of novel transcripts across all RNA types, accurate quantification of their expression levels, and characterization of their functional features.

Pan-RNA Identification and Profiling

It enables the discovery of novel transcripts across all RNA types, accurate quantification of their expression levels, and characterization of their functional features.

Disease Mechanism and Biomarker Discovery

By profiling multi-type RNA expression patterns in physiological and pathological contexts, it helps identify diagnostic, prognostic, and predictive biomarkers, and elucidate molecular mechanisms underlying various diseases.

Disease Mechanism and Biomarker Discovery

By profiling multi-type RNA expression patterns in physiological and pathological contexts, it helps identify diagnostic, prognostic, and predictive biomarkers, and elucidate molecular mechanisms underlying various diseases.

Transcriptional and Post-Transcriptional Regulation Elucidation

It facilitates the investigation of gene regulation networks, including transcriptional control, mRNA splicing, non-coding RNA-mediated regulation, and their crosstalk, thereby shedding light on complex regulatory landscapes.

Transcriptional and Post-Transcriptional Regulation Elucidation

It facilitates the investigation of gene regulation networks, including transcriptional control, mRNA splicing, non-coding RNA-mediated regulation, and their crosstalk, thereby shedding light on complex regulatory landscapes.

Therapeutic Target and Functional Study Tool

It serves as a foundation for exploring diverse RNA molecules as potential therapeutic targets. Also, it aids in functional studies to elucidate how different RNA species participate in cellular processes, development, and disease progression.

Therapeutic Target and Functional Study Tool

It serves as a foundation for exploring diverse RNA molecules as potential therapeutic targets. Also, it aids in functional studies to elucidate how different RNA species participate in cellular processes, development, and disease progression.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Demo Results

Image
Image
1/1
Statistics of differentially expressed genes

The statistics of up/down regulation of differentially expressed genes among all comparison groups.

Image
Image
1/1
Interaction of differentially expressed lncRNA’s target mRNA and differentially expressed mRNA

The elements are up_mRNA, up_lncRNA_target, down_lncRNA_target and down_mRNA from left to right.

Image
Image
1/1
circRNA-miRNA-gene counting and networks

Selecting the circRNA-miRNA-gene triplets in which circRNA, miRNA and genes are all differentially expressed and count their numbers in each comparison.

Image
Image
1/1
GO Enrichment Analysis Scatter Plot

The abscissa in the graph is the ratio of the differential gene number to the total number of differential genes on the GO Term, and the ordinate is GO Term.

Image
Image
1/1
Statistics of differentially expressed genes

The statistics of up/down regulation of differentially expressed genes among all comparison groups.

Image
Image
1/1
Interaction of differentially expressed lncRNA’s target mRNA and differentially expressed mRNA

The elements are up_mRNA, up_lncRNA_target, down_lncRNA_target and down_mRNA from left to right.

Image
Image
1/1
circRNA-miRNA-gene counting and networks

Selecting the circRNA-miRNA-gene triplets in which circRNA, miRNA and genes are all differentially expressed and count their numbers in each comparison.

Image
Image
1/1
GO Enrichment Analysis Scatter Plot

The abscissa in the graph is the ratio of the differential gene number to the total number of differential genes on the GO Term, and the ordinate is GO Term.

Demo Results

Image
Image
1/1
Statistics of differentially expressed genes

The statistics of up/down regulation of differentially expressed genes among all comparison groups.

Image
Image
1/1
Interaction of differentially expressed lncRNA’s target mRNA and differentially expressed mRNA

The elements are up_mRNA, up_lncRNA_target, down_lncRNA_target and down_mRNA from left to right.

Image
Image
1/1
circRNA-miRNA-gene counting and networks

Selecting the circRNA-miRNA-gene triplets in which circRNA, miRNA and genes are all differentially expressed and count their numbers in each comparison.

Image
Image
1/1
GO Enrichment Analysis Scatter Plot

The abscissa in the graph is the ratio of the differential gene number to the total number of differential genes on the GO Term, and the ordinate is GO Term.

Image
Image
1/1
Statistics of differentially expressed genes

The statistics of up/down regulation of differentially expressed genes among all comparison groups.

Image
Image
1/1
Interaction of differentially expressed lncRNA’s target mRNA and differentially expressed mRNA

The elements are up_mRNA, up_lncRNA_target, down_lncRNA_target and down_mRNA from left to right.

Image
Image
1/1
circRNA-miRNA-gene counting and networks

Selecting the circRNA-miRNA-gene triplets in which circRNA, miRNA and genes are all differentially expressed and count their numbers in each comparison.

Image
Image
1/1
GO Enrichment Analysis Scatter Plot

The abscissa in the graph is the ratio of the differential gene number to the total number of differential genes on the GO Term, and the ordinate is GO Term.

More Services

mRNA Sequencing
(mRNA Sequencing)
mRNA Sequencing
(mRNA Sequencing)
Total RNA Sequencing
(Total RNA Sequencing)
Total RNA Sequencing
(Total RNA Sequencing)
Metatranscriptome Sequencing
(Metatranscriptome Sequencing)
Metatranscriptome Sequencing
(Metatranscriptome Sequencing)

More Services

mRNA Sequencing
(mRNA Sequencing)
mRNA Sequencing
(mRNA Sequencing)
Total RNA Sequencing
(Total RNA Sequencing)
Total RNA Sequencing
(Total RNA Sequencing)
Metatranscriptome Sequencing
(Metatranscriptome Sequencing)
Metatranscriptome Sequencing
(Metatranscriptome Sequencing)
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Whole Transcriptome Sequencing

Comprehensive sequencing of the whole transcriptome to characterize coding and non-coding RNA expression, transcript structure, and regulatory complexity.
Request a Quote
(Whole Transcriptome Sequencing)
Request a Quote
(Whole Transcriptome Sequencing)
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Whole Transcriptome Sequencing is a powerful approach that profiles all types of RNA molecules in a biological sample, including mRNA, non-coding RNAs. It provides a comprehensive view of the transcriptome.

Novogene’s Whole Transcriptome Sequencing offers a holistic analysis of transcriptional activity. It enables the simultaneous identification and quantification of diverse RNA species, facilitates the exploration of complex RNA interactions and regulatory networks, allows in-depth investigation of how different RNA molecules coordinate in biological processes, disease development, and cellular functions, and enhances our understanding of the multi-layered regulation of gene expression.

Benefits of Whole Transcriptome Sequencing

Comprehensive Transcript Detection
Comprehensive Transcript Detection

Achieve simultaneous identification and quantification of all RNA types, including mRNA, lncRNA, sRNA, circRNA, with high sensitivity and precision.

Comprehensive Transcript Detection
Comprehensive Transcript Detection

Achieve simultaneous identification and quantification of all RNA types, including mRNA, lncRNA, sRNA, circRNA, with high sensitivity and precision.

Vast Research Expertise
Vast Research Expertise

Leverage extensive whole transcriptome sequencing project experience to support in-depth investigations into gene expression regulation, biological processes, and disease mechanisms.

Vast Research Expertise
Vast Research Expertise

Leverage extensive whole transcriptome sequencing project experience to support in-depth investigations into gene expression regulation, biological processes, and disease mechanisms.

Integrated Multi-Omics Analysis Solutions
Integrated Multi-Omics Analysis Solutions

Obtain all-round analysis covering transcript identification, expression profiling, differential expression analysis, functional annotation, and regulatory network construction across multiple RNA species.

Integrated Multi-Omics Analysis Solutions
Integrated Multi-Omics Analysis Solutions

Obtain all-round analysis covering transcript identification, expression profiling, differential expression analysis, functional annotation, and regulatory network construction across multiple RNA species.

Publication-Ready Data Curation
Publication-Ready Data Curation

Receive specialized bioinformatics support to generate publication-quality results, enabling seamless submission to high-impact scientific journals.

Publication-Ready Data Curation
Publication-Ready Data Curation

Receive specialized bioinformatics support to generate publication-quality results, enabling seamless submission to high-impact scientific journals.

Benefits of Whole Transcriptome Sequencing

Comprehensive Transcript Detection
Comprehensive Transcript Detection

Achieve simultaneous identification and quantification of all RNA types, including mRNA, lncRNA, sRNA, circRNA, with high sensitivity and precision.

Comprehensive Transcript Detection
Comprehensive Transcript Detection

Achieve simultaneous identification and quantification of all RNA types, including mRNA, lncRNA, sRNA, circRNA, with high sensitivity and precision.

Vast Research Expertise
Vast Research Expertise

Leverage extensive whole transcriptome sequencing project experience to support in-depth investigations into gene expression regulation, biological processes, and disease mechanisms.

Vast Research Expertise
Vast Research Expertise

Leverage extensive whole transcriptome sequencing project experience to support in-depth investigations into gene expression regulation, biological processes, and disease mechanisms.

Integrated Multi-Omics Analysis Solutions
Integrated Multi-Omics Analysis Solutions

Obtain all-round analysis covering transcript identification, expression profiling, differential expression analysis, functional annotation, and regulatory network construction across multiple RNA species.

Integrated Multi-Omics Analysis Solutions
Integrated Multi-Omics Analysis Solutions

Obtain all-round analysis covering transcript identification, expression profiling, differential expression analysis, functional annotation, and regulatory network construction across multiple RNA species.

Publication-Ready Data Curation
Publication-Ready Data Curation

Receive specialized bioinformatics support to generate publication-quality results, enabling seamless submission to high-impact scientific journals.

Publication-Ready Data Curation
Publication-Ready Data Curation

Receive specialized bioinformatics support to generate publication-quality results, enabling seamless submission to high-impact scientific journals.

Applications of Whole Transcriptome Sequencing

Whole transcriptome sequencing has emerged as a powerful tool to explore the comprehensive landscape of all RNA molecules in a biological system, with the following prominent applications:

Pan-RNA Identification and Profiling

It enables the discovery of novel transcripts across all RNA types, accurate quantification of their expression levels, and characterization of their functional features.

Pan-RNA Identification and Profiling

It enables the discovery of novel transcripts across all RNA types, accurate quantification of their expression levels, and characterization of their functional features.

Disease Mechanism and Biomarker Discovery

By profiling multi-type RNA expression patterns in physiological and pathological contexts, it helps identify diagnostic, prognostic, and predictive biomarkers, and elucidate molecular mechanisms underlying various diseases.

Disease Mechanism and Biomarker Discovery

By profiling multi-type RNA expression patterns in physiological and pathological contexts, it helps identify diagnostic, prognostic, and predictive biomarkers, and elucidate molecular mechanisms underlying various diseases.

Transcriptional and Post-Transcriptional Regulation Elucidation

It facilitates the investigation of gene regulation networks, including transcriptional control, mRNA splicing, non-coding RNA-mediated regulation, and their crosstalk, thereby shedding light on complex regulatory landscapes.

Transcriptional and Post-Transcriptional Regulation Elucidation

It facilitates the investigation of gene regulation networks, including transcriptional control, mRNA splicing, non-coding RNA-mediated regulation, and their crosstalk, thereby shedding light on complex regulatory landscapes.

Therapeutic Target and Functional Study Tool

It serves as a foundation for exploring diverse RNA molecules as potential therapeutic targets. Also, it aids in functional studies to elucidate how different RNA species participate in cellular processes, development, and disease progression.

Therapeutic Target and Functional Study Tool

It serves as a foundation for exploring diverse RNA molecules as potential therapeutic targets. Also, it aids in functional studies to elucidate how different RNA species participate in cellular processes, development, and disease progression.

Applications of Whole Transcriptome Sequencing

Whole transcriptome sequencing has emerged as a powerful tool to explore the comprehensive landscape of all RNA molecules in a biological system, with the following prominent applications:

Pan-RNA Identification and Profiling

It enables the discovery of novel transcripts across all RNA types, accurate quantification of their expression levels, and characterization of their functional features.

Pan-RNA Identification and Profiling

It enables the discovery of novel transcripts across all RNA types, accurate quantification of their expression levels, and characterization of their functional features.

Disease Mechanism and Biomarker Discovery

By profiling multi-type RNA expression patterns in physiological and pathological contexts, it helps identify diagnostic, prognostic, and predictive biomarkers, and elucidate molecular mechanisms underlying various diseases.

Disease Mechanism and Biomarker Discovery

By profiling multi-type RNA expression patterns in physiological and pathological contexts, it helps identify diagnostic, prognostic, and predictive biomarkers, and elucidate molecular mechanisms underlying various diseases.

Transcriptional and Post-Transcriptional Regulation Elucidation

It facilitates the investigation of gene regulation networks, including transcriptional control, mRNA splicing, non-coding RNA-mediated regulation, and their crosstalk, thereby shedding light on complex regulatory landscapes.

Transcriptional and Post-Transcriptional Regulation Elucidation

It facilitates the investigation of gene regulation networks, including transcriptional control, mRNA splicing, non-coding RNA-mediated regulation, and their crosstalk, thereby shedding light on complex regulatory landscapes.

Therapeutic Target and Functional Study Tool

It serves as a foundation for exploring diverse RNA molecules as potential therapeutic targets. Also, it aids in functional studies to elucidate how different RNA species participate in cellular processes, development, and disease progression.

Therapeutic Target and Functional Study Tool

It serves as a foundation for exploring diverse RNA molecules as potential therapeutic targets. Also, it aids in functional studies to elucidate how different RNA species participate in cellular processes, development, and disease progression.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity Number
(Agilent 5400)
Purity
(NanoDrop)
lncRNA library & small RNA libraryTotal RNA≥ 2.5 μg≥ 7.5, with flat
baseline
OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no genomic
contamination
lncRNA library & small RNA library & circRNA library≥ 4 μg

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina NovaSeq System
Read LengthlncRNA/circRNA library: paired-end 150bp
small RNA library: single-end 50bp
Recommended Data AmountlncRNA library: ≥ 40 million read pairs per sample
small RNA library: 10-20 million reads per sample
Content of Data AnalysisIndividual ncRNA analysis package
Network analysis between lncRNA vs miRNA, mRNA vs miRNA, circRNA vs miRNA, lncRNA-miRNA-mRNA, and circRNA-miRNA-mRNA

Project Workflow

Our Whole Transcriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Project Workflow

Demo Results

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Statistics of differentially expressed genes

The statistics of up/down regulation of differentially expressed genes among all comparison groups.

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Interaction of differentially expressed lncRNA’s target mRNA and differentially expressed mRNA

The elements are up_mRNA, up_lncRNA_target, down_lncRNA_target and down_mRNA from left to right.

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Image
1/1
circRNA-miRNA-gene counting and networks

Selecting the circRNA-miRNA-gene triplets in which circRNA, miRNA and genes are all differentially expressed and count their numbers in each comparison.

Image
Image
1/1
GO Enrichment Analysis Scatter Plot

The abscissa in the graph is the ratio of the differential gene number to the total number of differential genes on the GO Term, and the ordinate is GO Term.

Image
Image
1/1
Statistics of differentially expressed genes

The statistics of up/down regulation of differentially expressed genes among all comparison groups.

Image
Image
1/1
Interaction of differentially expressed lncRNA’s target mRNA and differentially expressed mRNA

The elements are up_mRNA, up_lncRNA_target, down_lncRNA_target and down_mRNA from left to right.

Image
Image
1/1
circRNA-miRNA-gene counting and networks

Selecting the circRNA-miRNA-gene triplets in which circRNA, miRNA and genes are all differentially expressed and count their numbers in each comparison.

Image
Image
1/1
GO Enrichment Analysis Scatter Plot

The abscissa in the graph is the ratio of the differential gene number to the total number of differential genes on the GO Term, and the ordinate is GO Term.

Demo Results

Image
Image
1/1
Statistics of differentially expressed genes

The statistics of up/down regulation of differentially expressed genes among all comparison groups.

Image
Image
1/1
Interaction of differentially expressed lncRNA’s target mRNA and differentially expressed mRNA

The elements are up_mRNA, up_lncRNA_target, down_lncRNA_target and down_mRNA from left to right.

Image
Image
1/1
circRNA-miRNA-gene counting and networks

Selecting the circRNA-miRNA-gene triplets in which circRNA, miRNA and genes are all differentially expressed and count their numbers in each comparison.

Image
Image
1/1
GO Enrichment Analysis Scatter Plot

The abscissa in the graph is the ratio of the differential gene number to the total number of differential genes on the GO Term, and the ordinate is GO Term.

Image
Image
1/1
Statistics of differentially expressed genes

The statistics of up/down regulation of differentially expressed genes among all comparison groups.

Image
Image
1/1
Interaction of differentially expressed lncRNA’s target mRNA and differentially expressed mRNA

The elements are up_mRNA, up_lncRNA_target, down_lncRNA_target and down_mRNA from left to right.

Image
Image
1/1
circRNA-miRNA-gene counting and networks

Selecting the circRNA-miRNA-gene triplets in which circRNA, miRNA and genes are all differentially expressed and count their numbers in each comparison.

Image
Image
1/1
GO Enrichment Analysis Scatter Plot

The abscissa in the graph is the ratio of the differential gene number to the total number of differential genes on the GO Term, and the ordinate is GO Term.

More Services

mRNA Sequencing
(mRNA Sequencing)
mRNA Sequencing
(mRNA Sequencing)
Total RNA Sequencing
(Total RNA Sequencing)
Total RNA Sequencing
(Total RNA Sequencing)
Metatranscriptome Sequencing
(Metatranscriptome Sequencing)
Metatranscriptome Sequencing
(Metatranscriptome Sequencing)

More Services

mRNA Sequencing
(mRNA Sequencing)
mRNA Sequencing
(mRNA Sequencing)
Total RNA Sequencing
(Total RNA Sequencing)
Total RNA Sequencing
(Total RNA Sequencing)
Metatranscriptome Sequencing
(Metatranscriptome Sequencing)
Metatranscriptome Sequencing
(Metatranscriptome Sequencing)
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