Circular RNA Sequencing

Transcriptome-wide profiling of circular RNAs to reveal noncoding regulatory mechanisms and RNA expression patterns across biological conditions.

Circular RNA (circRNA) is a highly stable molecule of non-coding RNA, in the form of a covalently closed loop that lacks the 5′-end caps and the 3′-poly(A) tails. The circular structure grants circRNAs resistance against exonuclease digestion, a characteristic that can be exploited in library construction.

Novogene’s Circular RNA Sequencing (circRNA-seq) uses next-generation sequencing (NGS) technology to support a wide range of investigations focused on circRNA. The regulatory function of circRNAs may be involved in many biological processes, such as regulation of gene expression by acting as miRNA sponges, shuttling of miRNAs, and regulation of protein synthesis.

Benefits of CircRNA Sequencing

High-Resolution circRNA Detection

Achieve sensitive and specific identification of circular RNAs, including rare and novel circRNAs, with high sequencing depth and accuracy.

Extensive circRNA-Seq Research Experience

Leverage a wealth of circRNA-seq project experience to support in-depth studies on circRNA biology and disease mechanisms.

Comprehensive circRNA-Seq Analysis Solutions

Obtain all-round analysis covering circRNA identification, quantification, differential expression, back-splice junction detection and analysis, and functional prediction.

Publication-Oriented Bioinformatics Support

Receive professional bioinformatics support to generate publication-ready results, facilitating high-impact journal submissions.

Applications of Circular RNA Sequencing

Circular RNA sequencing (circRNA-seq) has emerged as a powerful tool to explore the diverse roles of circRNAs with the following prominent applications:

Comprehensive Identification and Characterization

It enables the discovery of novel circRNAs, accurate quantification of their expression levels, and characterization of their structural features.

Disease and Biomarker Discovery

By profiling circRNA expression patterns in different physiological and pathological states, it enables the identification of circRNAs as diagnostic, prognostic, and predictive biomarkers for diseases.

Regulatory Function Exploration

It facilitates the investigation of circRNAs’ regulatory mechanisms, thereby shedding light on post-transcriptional gene regulation networks.

Therapeutic and Functional Study Tool

It serves as a foundation for exploring circRNAs as potential therapeutic targets. Also, it supports functional studies aimed at elucidating circRNA roles in cellular processes.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

The workflow of the circRNA-seq starts with sample preparation and quality control. The ribosomal RNA (rRNA) is depleted for target transcript enrichment. Subsequently, the linear RNA is enzymatically digested and the fragmented RNA is reverse-transcribed into cDNA. Strand-specific libraries are prepared, and the sequencing is performed using a paired-end 150bp strategy on the Illumina sequencing platform. The downstream processing follows the well-validated, Novogene bioinformatics pipeline, which guarantees the highest quality results. Customized bioinformatics solutions are available upon request.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Demo Results

1/1

Distribution of circRNAs in the chromosome

Density statistics are conducted for all circRNAs of each sample compared to each chromosome on the genome, and circRNAs distribution on each chromosome was plotted by circos.

1/1

circRNAs expression level distribution

The expression values of all circRNAs in each sample are calculated as TPM, and the overall gene expression pattern of the sample is displayed through the TPM-based box plot.

1/1

Differential circRNAs results

The x-axis shows the fold change in circRNAs expression between different samples, and the y-axis shows the statistical significance of differences. Statistically significant differentially expressed circRNAs are indicated by red dots.

1/1

Histogram of source gene GO enrichment

The x-axis represents GO terms; the y-axis represents the significance level (−log₁₀(p-value)) of GO term enrichment.

1/1

KEGG enrichment scatter plot

The x-axis shows the ratio of differentially expressed genes mapped to a given KEGG pathway to the total number of differentially expressed genes; the y-axis lists the corresponding KEGG pathways.

Demo Results

1/1

Distribution of circRNAs in the chromosome

Density statistics are conducted for all circRNAs of each sample compared to each chromosome on the genome, and circRNAs distribution on each chromosome was plotted by circos.

1/1

circRNAs expression level distribution

The expression values of all circRNAs in each sample are calculated as TPM, and the overall gene expression pattern of the sample is displayed through the TPM-based box plot.

1/1

Differential circRNAs results

1/1

Histogram of source gene GO enrichment

The x-axis represents GO terms; the y-axis represents the significance level (−log₁₀(p-value)) of GO term enrichment.

1/1

KEGG enrichment scatter plot

The x-axis shows the ratio of differentially expressed genes mapped to a given KEGG pathway to the total number of differentially expressed genes; the y-axis lists the corresponding KEGG pathways.

More Services

Total RNA Sequencing

Small RNA Sequencing

Whole Transcriptome Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

Circular RNA Sequencing

Transcriptome-wide profiling of circular RNAs to reveal noncoding regulatory mechanisms and RNA expression patterns across biological conditions.

Benefits of CircRNA Sequencing

High-Resolution circRNA Detection

Achieve sensitive and specific identification of circular RNAs, including rare and novel circRNAs, with high sequencing depth and accuracy.

Extensive circRNA-Seq Research Experience

Leverage a wealth of circRNA-seq project experience to support in-depth studies on circRNA biology and disease mechanisms.

Comprehensive circRNA-Seq Analysis Solutions

Obtain all-round analysis covering circRNA identification, quantification, differential expression, back-splice junction detection and analysis, and functional prediction.

Publication-Oriented Bioinformatics Support

Receive professional bioinformatics support to generate publication-ready results, facilitating high-impact journal submissions.

Applications of Circular RNA Sequencing

Circular RNA sequencing (circRNA-seq) has emerged as a powerful tool to explore the diverse roles of circRNAs with the following prominent applications:

Comprehensive Identification and Characterization

It enables the discovery of novel circRNAs, accurate quantification of their expression levels, and characterization of their structural features.

Disease and Biomarker Discovery

Regulatory Function Exploration

It facilitates the investigation of circRNAs’ regulatory mechanisms, thereby shedding light on post-transcriptional gene regulation networks.

Therapeutic and Functional Study Tool

It serves as a foundation for exploring circRNAs as potential therapeutic targets. Also, it supports functional studies aimed at elucidating circRNA roles in cellular processes.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number (Agilent 5400)	Purity (NanoDrop)
circRNA Library	Total RNA	≥ 2 μg	Animal ≥ 7, Plant ≥ 6.5, with smooth baseline	OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq System
Read Length	Paired-end 150bp
Recommended Sequencing Depth	≥ 40 million read pair per sample
Standard Data Analysis	Data Quality Control circRNA Identification Transcript Expression Quantification & Differential Expression Analysis Functional Enrichment Analysis CircRNA Target Gene Prediction

Project Workflow

Demo Results

1/1

Distribution of circRNAs in the chromosome

Density statistics are conducted for all circRNAs of each sample compared to each chromosome on the genome, and circRNAs distribution on each chromosome was plotted by circos.

1/1

circRNAs expression level distribution

The expression values of all circRNAs in each sample are calculated as TPM, and the overall gene expression pattern of the sample is displayed through the TPM-based box plot.

1/1

Differential circRNAs results

1/1

Histogram of source gene GO enrichment

The x-axis represents GO terms; the y-axis represents the significance level (−log₁₀(p-value)) of GO term enrichment.

1/1

KEGG enrichment scatter plot

The x-axis shows the ratio of differentially expressed genes mapped to a given KEGG pathway to the total number of differentially expressed genes; the y-axis lists the corresponding KEGG pathways.

Demo Results

1/1

Distribution of circRNAs in the chromosome

Density statistics are conducted for all circRNAs of each sample compared to each chromosome on the genome, and circRNAs distribution on each chromosome was plotted by circos.

1/1

circRNAs expression level distribution

The expression values of all circRNAs in each sample are calculated as TPM, and the overall gene expression pattern of the sample is displayed through the TPM-based box plot.

1/1

Differential circRNAs results

1/1

Histogram of source gene GO enrichment

The x-axis represents GO terms; the y-axis represents the significance level (−log₁₀(p-value)) of GO term enrichment.

1/1

KEGG enrichment scatter plot

The x-axis shows the ratio of differentially expressed genes mapped to a given KEGG pathway to the total number of differentially expressed genes; the y-axis lists the corresponding KEGG pathways.

More Services

Total RNA Sequencing

Small RNA Sequencing

Whole Transcriptome Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.