Introduction:

Single-cell sequencing is an emerging technique of high-throughput sequencing and analyzing genomics, transcriptome, and epigenomics at single-cell level. The development of sequencing technology provides fertile ground, making it a popular research field in life sciences and it is also more widely used in basic medicine, clinical translation research and Oncology. Oncology is a specialized field within medicine dedicated to the comprehensive study, diagnosis, treatment, and prevention of cancer. The cutting-edge scRNA-seq technique enables researchers to dissect the heterogeneity within tumor tissues, providing insights into the diverse cell populations, their interactions, and the molecular mechanisms underlying cancer progression.