Introduction:

Single-cell RNA-sequencing (scRNA-seq) uses next-generation sequencing (NGS) technologies to examine the sequence information contained within single cells. This advancement in technology enables us to examine the genome and transcriptome and provide other multi-omics information,giving us a better understanding of the function ofan individual cell and uncovering mechanisms that could otherwise go unnoticed when examining multiple cells at once. Single-cell sequencing can also reveal differences between populations of cells and study cellular evolutionary relationships.This makes it a useful tool for studying cellular function as well as for examining the development of tissues and tumors.