Introduction:

Isoform sequencing (Iso-Seq) is a long-read sequencing technique developed by PacBio that uses Single-Molecule Real-Time (SMRT) technology to capture full-length transcript isoforms without the need for transcript assembly. By utilizing Circular Consensus Sequencing (CCS) and high-quality long reads (HiFi reads), Iso-Seq provides high-resolution insights into transcriptome complexity, enabling the discovery of novel isoforms, alternative splicing events, and precise annotation of genome.