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    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

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    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on PacBio Sequencer
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  3. Eukaryotic mRNA-seq Sample Preparation — Getting Qualified Total RNA Samples

Eukaryotic mRNA-seq Sample Preparation — Getting Qualified Total RNA Samples

RNA is a single-stranded nucleic acid polymer of the four nucleotides A, C, G, and U, joined through a backbone of alternating phosphate and ribose residues, converting information from DNA into proteins or to serve direct roles in cells, like rRNA, tRNA, etc. Among all kinds of RNAs in cells, messenger RNA (mRNA) is a molecular that carries codes from the DNA in the nucleus to the sites of protein synthesis in the cytoplasm (the ribosomes).

Novogene’s mRNA-Seq, based on state-of-the-art Illumina NovaSeq platforms, is commonly used to profile the snapshot of mRNA or even the entire transcriptome at a given moment in time. It offers comprehensive solutions for analyzing gene expression and differential gene expression among sample groups, as well as for identification of gene structure variations, such as alternative splicing, gene fusion events, etc.

High-quality total RNA as starting material for an mRNA-seq project is vital to the successful mining of bioinformatics information from the sequencing data. Novogene conducts stringent Quality Control to help you select high-quality total RNA samples for sequencing, avoiding any potential issues downstream. What’s more, in this webinar we also share with you our extensive experience as a guideline in sample preparation from tissues.

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Services
Whole Genome SequencingDe novo SequencingAmplicon SequencingShotgun Metagenomic SequencingDirected DNA Methylation Sequencing (DM-Seq)mRNA SequencingSingle Cell Gene ExpressionVisium HD Spatial Gene ExpressionXenium In Situ Spatial TranscriptomeOlink ProteomicsUntargeted Metabolomics
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NovoMagic Bioinformatics Analysis ToolCustomer Service SystemFalcon Intelligent Delivery Platform
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Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
Novogene AMEA
  • Novogene AMEA
  • Genomics
    • Human Whole Genome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Microbial Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial De novo Sequencing
    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing
    • Whole Exome Sequencing
    Transcriptomics
    • mRNA Sequencing
    • Total RNA Sequencing
    • Full-Length Transcriptome Sequencing
    • Whole Transcriptome Sequencing
    • Small RNA Sequencing
    • Circular RNA Sequencing
    • Metatranscriptome Sequencing
    • Prokaryotic RNA Sequencing
    Single Cell & Spatial Omics
    • Single Cell Gene Expression
    • Single Cell Immune Profiling Sequencing
    • Single Cell Long Read Transcriptome
    • Visium HD Spatial Gene Expression
    • Stereo-Seq Spatial Gene Expression
    • Xenium In Situ Spatial Transcriptome
    Epigenomics
    • Whole Genome Bisulfite Sequencing (WGBS)
    • Directed DNA Methylation Sequencing (DM-Seq) NEW
    • Reduced Representation Bisulfite Sequencing (RRBS)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on PacBio Sequencer
    Proteomics and Metabolomics
    • Olink Proteomics
    • Quantitative Proteomics
    • Untargeted Metabolomics
  • PromotionsPromotions
    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Brochures
    • Case Studies
    • Webinar
    • Blog
    • Sample Guidelines
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • Our Locations
    • News
    • Careers
  • Contact UsContact Us
  1. Home
  2. Resources
  3. Eukaryotic mRNA-seq Sample Preparation — Getting Qualified Total RNA Samples

Eukaryotic mRNA-seq Sample Preparation — Getting Qualified Total RNA Samples

RNA is a single-stranded nucleic acid polymer of the four nucleotides A, C, G, and U, joined through a backbone of alternating phosphate and ribose residues, converting information from DNA into proteins or to serve direct roles in cells, like rRNA, tRNA, etc. Among all kinds of RNAs in cells, messenger RNA (mRNA) is a molecular that carries codes from the DNA in the nucleus to the sites of protein synthesis in the cytoplasm (the ribosomes).

Novogene’s mRNA-Seq, based on state-of-the-art Illumina NovaSeq platforms, is commonly used to profile the snapshot of mRNA or even the entire transcriptome at a given moment in time. It offers comprehensive solutions for analyzing gene expression and differential gene expression among sample groups, as well as for identification of gene structure variations, such as alternative splicing, gene fusion events, etc.

High-quality total RNA as starting material for an mRNA-seq project is vital to the successful mining of bioinformatics information from the sequencing data. Novogene conducts stringent Quality Control to help you select high-quality total RNA samples for sequencing, avoiding any potential issues downstream. What’s more, in this webinar we also share with you our extensive experience as a guideline in sample preparation from tissues.

ServicesServices menu

SupportSupport menu

CompanyCompany menu

Services
Whole Genome SequencingDe novo SequencingAmplicon SequencingShotgun Metagenomic SequencingDirected DNA Methylation Sequencing (DM-Seq)mRNA SequencingSingle Cell Gene ExpressionVisium HD Spatial Gene ExpressionXenium In Situ Spatial TranscriptomeOlink ProteomicsUntargeted Metabolomics
Support
NovoMagic Bioinformatics Analysis ToolCustomer Service SystemFalcon Intelligent Delivery Platform
Company
About UsOur LocationsOur PlatformsNewsCareersContact Us
LinkedInLinkedIn hoverYouTubeYouTube hoverXX hover
Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
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