RNA immunoprecipitation sequencing (RIP-seq or RIP sequencing) is a high-throughput RNA sequencing technology dedicated to the systematic analysis of protein-RNA interactions. It enables genome-wide characterization of the binding events between target proteins and RNA molecules, thereby elucidating the regulatory mechanisms of RNA-binding proteins (RBPs) in critical biological processes such as gene expression, RNA splicing, RNA stability, subcellular localization, and translational regulation.
Notably, this technique supports the simultaneous detection of diverse RNA species in a single experimental run, including mRNAs and non-coding RNAs (ncRNAs). When integrated with complementary technologies such as ChIP-seq and ATAC-seq, RIP-seq facilitates multi-omics analyses that yield comprehensive and in-depth insights into the molecular underpinnings of biological activities.
